文献类型：期刊文献

英文题名：Cloning, expression of Vibrio alginolyticus outer membrane protein-OmpU gene and its potential application as vaccine in crimson snapper, Lutjanus erythropterus Bloch

作者：Cai, S. H.[1,2,3];Lu, Y. S.[1,2,3];Wu, Z. H.[2,3,4];Jian, J. C.[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou, Guangdong, Peoples R China

年份：2013

卷号：36

期号：8

起止页码：695

外文期刊名：JOURNAL OF FISH DISEASES

收录：SCI-EXPANDED(收录号：WOS:000321517700002)、、Scopus(收录号：2-s2.0-84880311823)、WOS

基金：This work was supported by National Program on Key Basic Research Project (2009CB118704), the 973 Program Earlier Research Project (2011CB111601), Foundation for Distinguished Young Talents in Higher Education of Guangdong Province (No. LYM08063) and National Science Foundation of Guangdong Province (No. 9452408801002439 and No. S2011010000257). We thank all the people who have dedicated time to the experiments.

语种：英文

外文关键词：immunogenicity; Lutjanus erythropterus; OmpU; vaccine; Vibrio alginolyticus

外文摘要：The outer membrane proteins of the marine aquatic animal pathogen, Vibrio alginolyticus, play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In this study, the gene encoding an outer membrane protein-OmpU was cloned and expressed in Escherichia coli. Polyclonal antibodies were raised in rabbits against the purified recombinant OmpU, and the reaction of the antibody was confirmed by Western blotting using the isolated OmpU and the recombinant OmpU of V. alginolyticus. To analyze the immunogenicity of the recombinant OmpU, crimson snapper, Lutjanus erythropterus Bloch, were immunized by intraperitoneal injection, and antibody response was assessed by the enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the recombinant OmpU produced an observable antibody response in all sera of the vaccinated fish. The vaccinated fish were challenged by virulent V. alginolyticus and observed to have high resistance to infection. These results indicate that the recombinant OmpU is an effective vaccine candidate against V. alginolyticus in L. erythropterus.