文献类型：期刊文献

英文题名：Heat Stress Affects Jejunal Immunity of Yellow-Feathered Broilers and Is Potentially Mediated by the Microbiome

作者：Liu, Wen-Chao[1];Huang, Meng-Yi[1];Balasubramanian, Balamuralikrishnan[2];Jha, Rajesh[3]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Anim Sci, Zhanjiang, Peoples R China;[2]Sejong Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Seoul, South Korea;[3]Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Human Nutr Food & Anim Sci, Honolulu, HI 96822 USA

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PHYSIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000806848400001)、、Scopus(收录号：2-s2.0-85131816338)、WOS

基金：Funding This research was funded by the National Nature Science Foundation of China (32002196).

语种：英文

外文关键词：gene expression; gut microbiota; heat stress; intestinal immunity; yellow-feathered broilers

外文摘要：In the perspective of the global climate change leading to increasing temperature, heat stress (HS) has become a severe issue in broiler production, including the indigenous yellow-feathered broilers. The present study aimed to investigate the effects of HS on jejunal immune response, microbiota structure and their correlation in yellow-feathered broilers. A total of forty female broilers (56-days-old) were randomly and equally divided into normal treatment group (NT group, 21.3 +/- 1.2 degrees C, 24 h/day) and HS group (32.5 +/- 1.4 degrees C, 8 h/day) with five replicates of each for 4 weeks feeding trial. The results showed that HS exposure increased the contents of TNF-alpha, IL-1 beta, and IL-6 in jejunal mucosa (p < 0.05). The HS exposure up-regulated the relative fold changes of NF-kappa B, TNF-alpha, IL-1 beta, and IL-6 (p < 0.01) while down-regulated the relative fold change of IFN-gamma in jejunal mucosa (p < 0.05). Meanwhile, HS had no significant impacts on alpha diversity of jejunal microbiota such as Simpson, Chao1 richness estimator (Chao 1), abundance-based coverage estimators (ACE), and Shannon index (p > 0.10). Broilers exposed to HS reduced the jejunal microbial species number at the class and order level (p < 0.05). Moreover, HS decreased the relative abundance of Ruminococcus, Bdellovibrio, and Serratia at the genus level in jejunum (p < 0.05). At the phylum level, four species of bacteria (Bacteroidetes, Cyanobacteria, Thermi, and TM7) were significantly associated with immune-related genes expression (p < 0.05). At the genus level, ten species of bacteria were significantly correlated with the expression of immune-related genes (p < 0.05), including Caulobacteraceae, Actinomyces, Ruminococcaceae, Thermus, Bdellovibrio, Clostridiales, Sediminibacterium, Bacteroides, Sphingomonadales and Ruminococcus. In particular, the microbial with significantly different abundances, Ruminococcus and Bdellovibrio, were negatively associated with pro-inflammatory cytokines expression (p < 0.05). These findings demonstrated that HS exposure promoted the production of pro-inflammatory cytokines in yellow-feathered broilers' jejunum. The detrimental effects of HS on jejunal immune response might be related to dysbiosis, especially the reduced levels of Ruminococcus and Bdellovibrio.