文献类型：期刊文献

英文题名：Establishment of an astrocyte-like cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the blood-brain barrier

作者：Li, Yuan[1,2];Huang, Yu[1,3];Cai, Jia[1,3];Jiang, Dongneng[1];Jian, Ji-chang[1,3];Lu, Yi-shan[1,2,3];Wang, Bei[1,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & H, Key Lab Control Dis Aquat Anim,Guangdong Higher E, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen Inst, Shenzhen, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang, Peoples R China

年份：2022

卷号：45

期号：10

起止页码：1451

外文期刊名：JOURNAL OF FISH DISEASES

收录：SCI-EXPANDED(收录号：WOS:000816749100001)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 32002426, U20A2065, 32073006) and Guangdong Basic and Applied Basic Research Foundation (2021A1515110214)

语种：英文

外文关键词：astrocyte; blood-brain barrier; cell line; Oreochromis niloticus; trans-well

外文摘要：A new cell line was established from the brain of a cultured fish, tilapia (Oreochromis niloticus), designated as TA-02 (Tilapia Astrocyte clone 02 cell line). The TA-02 cells are grown for 300 days in an L-15 medium supplemented with 10% fetal bovine serum (FBS). This cell line showed excellent proliferative capacity and expressed various neuroglial cell markers, including SOX2, SOX10, Hes1, Notch1, Occludin, E-cadherin, and GFAP. In addition, TA-02 cells were susceptible to Tilapia Lake Virus (TiLV) as demonstrated by the presence of a severe cytopathic effect (CPE), virus particle in a transmission electron microscope (TEM), and PCR positive signal. Bacterial cytotoxicity studies showed that Streptococcus agalactiae was toxic to TA-02 cells. When co-culture with trans-well, TA-02 exhibited prominent barrier properties, manifested by tight intercellular junctions and increased trans-endothelial electrical resistance (TEER). In addition, the barrier is effective against Escherichia coli (non-meningitis pathogenic bacteria). In contrast, S. agalactiae (meningitis pathogenic bacteria) can pass through the membrane comprising the cells in the trans-well insert. The newly established TA-02 cell line provided a valuable tool for virus pathogenesis and a vitro model of the fish blood-brain barrier.