文献类型：期刊文献

英文题名：Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper (4Epinephelus fuscoguttatus x dEpinephelus lanceolatus)

作者：Amoah, Kwaku[1,2,3];Tan, Beiping[1,2,3];Zhang, Shuang[1,2,3];Chi, Shuyan[1,2,3];Yang, Qihui[1,2,3];Liu, Hongyu[1,2,3];Yang, Yuanzhi[1];Zhang, Haitao[3];Dong, Xiaohui[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Engn, Zhanjiang 524088, Guangdong, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Techn, Zhanjiang 524000, Guangdong, Peoples R China

年份：2023

卷号：14

起止页码：163

外文期刊名：ANIMAL NUTRITION

收录：SCI-EXPANDED(收录号：WOS:001037485100001)、、WOS

基金：This study was supported financially by the General Program of Natural Science Foundation of Guangdong Province (2021A1515011165) , the Department of Education of Guangdong Province (2021ZDZX4005) , the National Natural Science Foundation of China (31972808) , the Research and Demonstration of Precision Functional Compound Feed Technology of Major Cultured Fishes and Shrimps in South China (2021B0202050002) , and the China Agriculture Research System of MOF and MARA (CARS-47) .

语种：英文

外文关键词：Host-associated probiotics; Immune and antioxidant-biochemical & nbsp; responses; Gut microbiota; Immune-related gene expression; Vibrio harveyi; Hybrid grouper (4Epinephelus fuscoguttatus x dE; lanceolatus)

外文摘要：Several reports have revealed the vital role that probiotics play in fish growth and health. However, few works are available for host gut-derived probiotics on the growth, immunity, and gut microbiota of fish, especially in hybrid grouper (4Epinephelus fuscoguttatus x dEpinephelus lanceolatus) due to their isolation difficulty and functional verification. This study aimed at assessing 3 host gut-derived Bacillus species' effects on the growth, immune and antioxidant-biochemical responses, haematological parameters, intestinal morphology, immune-related gene expression, gut microbiota, and disease resistance against Vibrio harveyi in hybrid grouper. A total of 480 hybrid grouper (initial weight = 9.03 & PLUSMN; 0.02 g) were randomly allotted into 4 groups, namely, the group fed a basal diet without probiotic inclusion (control, B0), the group fed the basal diet with Bacillus velezensis GPSAK4 (BV), the group fed the basal diet with Bacillus subtilis GPSAK9 (BS), and the group fed the basal diet with Bacillus tequilensis GPSAK2 (BT) strains at 1.0 x 109 CFU/g. After a 6-week feeding trial, the results revealed significant improvements (P < 0.05) in the growth performance, whole fish-body proximate composition, blood haematological parameters, serum, liver, and intestinal biochemical indexes, intestinal morphology, and protection against V. harveyi pathogen in the probiotic-treated groups compared with the untreated. Additionally, the expressions of intestinal tight junction genes (occludin and ZO1), pro- and anti-inflammatory genes, including IL1b, IL6, IL8, TNFa, MyD88, IL10, and TGFb, were upregulated (P < 0.05) after Bacillus species administration. Host gut-derived Bacillus supplementation shaped the gut microbiota by significantly increasing (P < 0.05) the relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria (except the BS group), Acidobacteria (except the BT group), Cyanobacteria (except the BV and BT groups), and Verrucomicrobia phyla, as well as known beneficial genera (Romboutsia, Turicibacter, Epulopiscium, Clostridium_sensu_stricto 1 and 13, Lactobacillus, and Bacillus), but significantly decreased (P < 0.05) the abundance of Firmicutes, Chloroflexi, and Fusobacteria phyla, and purported pathogenic genera (Staphylococcus and Photobacterium) compared with the control group. Collectively, the results suggest that B. velezensis GPSAK4, B. subtilis GPSAK9 (especially this strain), B. tequilensis GPSAK2 dietary supplementation at 1.0 x 109 CFU/g has positive effects on the intestinal health of hybrid grouper via microbial composition modulation, thus enhancing the assimilation and absorption of nutrients to boost fish growth, immunity, and disease resistance.& COPY; 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).