文献类型：期刊文献

中文题名：牡蛎低分子肽LOPs对短期免疫抑制小鼠的免疫调节作用

英文题名：Immunomodulatory Effects of Low Molecular Weight Oyster Peptides in Short-term Immunosuppressed Mice

作者：李晋祯[1,2];郑惠娜[1,2,3,4];任鼎鼎[1,2];杨文[1,2];曹文红[1,2,3,4];林海生[1,2,3,4];秦小明[1,2,3,4];章超桦[1,2,3,4]

机构：[1]广东海洋大学深圳研究院,广东深圳518108;[2]广东海洋大学食品科技学院,广东湛江524088;[3]国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东湛江524088;[4]海洋食品精深加工关键技术省部共建协同创新中心大连工业大学,辽宁大连116034

年份：2022

卷号：22

期号：10

起止页码：143

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD、、CSCD2021_2022、EI(收录号：20225013227071)、Scopus、北大核心、CSCD、北大核心2020

基金：广东省现代农业产业技术体系创新团队项目(2021KJ146);现代农业产业技术体系专项(CARS-49);深圳市大鹏新区科技创新和产业发展专项(KJYF202101-07);国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD091102)。

语种：中文

中文关键词：牡蛎低分子肽;环磷酰胺;免疫抑制;免疫调节

外文关键词：low-molecular-weight oyster peptides;cyclophosphamide;immunosuppression;immunomodulatory

中文摘要：目的:研究牡蛎低分子肽(LOPs)对短期免疫抑制小鼠的免疫调节作用。方法:120只雄性KM小鼠按照体质量随机分为空白组、环磷酰胺模型组、乳清蛋白对照组及LOPs低、中、高剂量组。实验开始1~3 d,空白组每天腹腔注射生理盐水10 mL/kg bw,其余组每天腹腔注射等体积环磷酰胺80 mg/kg bw,建立短期免疫抑制小鼠模型。第4天起,乳清蛋白组经口灌胃给予0.4 g/kg bw乳清蛋白,LOPs低、中、高剂量组分别经口灌胃给予0.2,0.4,0.8 g/kg bw的LOPs。空白对照组和模型组经口灌胃等体积蒸馏水。连续灌胃27 d后,测定小鼠体质量、免疫脏器质量及其组织形态、血清溶血素、细胞因子和免疫球蛋白。结果:与模型组相比,LOPs组有助于修复小鼠脾脏和胸腺组织形态学结构的损伤,增强血清溶血素水平,提高白细胞介素-2、γ-干扰素、免疫球蛋白质量浓度。结论:LOPs对环磷酰胺短期免疫抑制小鼠具有一定的免疫调节作用,具有作为特殊医学用途配方食品基料的潜在应用价值。

外文摘要：Objective: To explore the immunomodulatory effects of low-molecular-weight oyster peptides(LOPs) on shortterm immunosuppressed mice. Method: One hundred and twenty male KM mice were randomly divided into control, cyclophosphamide model, whey protein and low-, medium-and high-dose LOPs groups according to their body mass. In the first three days of the experiment, the control group was injected intraperitoneally with saline 10 mL/kg bw per day,and the rest of the groups were injected intraperitoneally with an equal volume of cyclophosphamide 80 mg/kg bw per day to establish a short-term immunosuppressed mice model. Subsequently, 0.4 g/kg bw of whey protein was given by oral gavage to whey protein group, 0.2, 0.4, and 0.8 g/kg bw of LOPs were given by oral gavage to low, medium and high dose groups, respectively, and an equal volume of distilled water was given by oral gavage to control and model group,and body mass, immune organ mass and its histomorphology, serum lysozyme, cytokines and immunoglobulins were measured after 27 d of gavage. Result: Compared with the model group, the LOPs group helped repair damage to the morphological structures of spleen and thymus tissues, enhanced serum hemolysin levels, and increased interleukin-2, gamma-interferon, and immunoglobulin mass concentrations in mice. Conclusion: LOPs have immunomodulatory effects on cyclophosphamide of short-term immunosuppressed mice and have potential application as a base for special medical use formulae.