文献类型：期刊文献

英文题名：Immune effect of Vibrio harveyi formalin-killed cells vaccine combined with chitosan oligosaccharide and astragalus polysaccharides in ♀Epinephelus fuscoguttatus x ♂Epinephelus lanceolatus

作者：Wei, Guangben[1,2,3,4];Cai, Shuanghu[1,2,3,4];Wu, Yuanzhi[1,2,3,4];Ma, Shaohong[1,2,3,4];Huang, Yucong[1,2,3,4]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Fisheries Coll, Zhanjiang, Peoples R China;[2]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China;[3]Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[4]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Peoples R China

年份：2020

卷号：98

起止页码：186

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000516880900021)、、WOS

基金：This work was funded by the National Key R&D Program of China (No. 2018YFD0900501), National Science Foundation of Guangdong Province (No. 2015A030308020) and Science and Technology Planning Project of Guangdong Province (No. 2017B020204004).

语种：英文

外文关键词：Vibrio harveyi; Formalin-inactivated cells vaccine; Immune response; Chitosan oligosaccharide; Astragalus polysaccharides

外文摘要：Vibrio harveyi is the pathogen causing vibriosis in marine-cultured animals, leading to massive deaths in farmed grouper around the world. It is urgent to develop an effective vaccine to prevent vibriosis. In the previous study, we developed a V. harveyi formalin-killed cells vaccine (FKC), and sought an effective adjuvant for enhancing the immune efficacy of vaccine. In this study, we aimed to evaluate the immune responses and protective effect of FKC combined with chitosan oligosaccharide (COS) or Astragalus polysaccharides (APS) in the pearl gentian grouper 9 Epinephelus fuscoguttatus x male E. lanceolntus. The results indicated the vaccine triggered a remarkably higher expression levels of IL-1 beta, IL-16, TNF-alpha, MHC-I alpha and IgM in the kidney and spleen of groupers post-vaccination. Antibody titers, lysozyme, catalase, superoxide dismutase and total protein were significantly elevated in the vaccinated fish compared with those in the control. The experimental groupers were challenged intraperitoneally by V. harveyi at 35 d post-vaccination, and the relative percentage of survival (RPS) of group FKC + COS, FKC + APS, COS, APS and FKC were 80%, 72%, 52%, 47% and 55%, respectively. These results demonstrated COS and APS was the potential adjuvants for FKC against V. harveyi in aquaculture.