文献类型：期刊文献

英文题名：Comparative transcriptomics and host-specific parasite gene expression profiles inform on drivers of proliferative kidney disease

作者：Faber, Marc[1];Shaw, Sophie[2];Yoon, Sohye[1,8];Alves, Eduardo de Paiva[2,3];Wang, Bei[1,4];Qi, Zhitao[1,5];Okamura, Beth[6];Hartikainen, Hanna[7];Secombes, Christopher J.[1];Holland, Jason W.[1]

机构：[1]Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland;[2]Univ Aberdeen, Ctr Genome Enabled Biol & Med, Aberdeen AB24 2TZ, Scotland;[3]Aigenpulse Com, 115J Olymp Ave,Milton Pk, Abingdon OX14 4SA, Oxon, England;[4]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim, Guangdong Higher Educ Inst,Coll Fishery, Zhanjiang, Peoples R China;[5]Yancheng Inst Technol, Key Lab Biochem & Biotechnol Marine Wetland Jiang, Yancheng 224051, Jiangsu, Peoples R China;[6]Nat Hist Museum, Dept Life Sci, London SW7 5BD, England;[7]Univ Nottingham, Sch Life Sci, Nottingham NG7 2RD, England;[8]Univ Queensland, Genome Innovat Hub, Brisbane, Qld 4072, Australia

年份：2021

卷号：11

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：SCI-EXPANDED(收录号：WOS:000733298800011)、、Scopus(收录号：2-s2.0-85099826511)、WOS

基金：This study was supported financially by the Biotechnology and Biological Sciences Research Council (BBSRC-1087-CS), a Swiss National Science Foundation Sinergia Project (CRS113 147649), a Ph.D. studentship to Marc Faber from the EU H2020 (H2020-SFS-10a-2014) program (ParaFishControl; 634429) and the Natural History Museum, London. We would like to thank Christopher Saunders-Davies of Test Valley Trout Ltd and Oliver Robinson of the British Trout Association for provision of fish and sampling facilities. Dr Daniel MacQueen of the Roslin Institute, University of Edinburgh, for provision of rainbow trout transcriptome assemblies. This publication reflects the views only of the authors, and the European Commission cannot be held responsible for any use which may be made of the information contained therein.

语种：英文

外文摘要：The myxozoan parasite, Tetracapsuloidesbryosalmonae has a two-host life cycle alternating between freshwater bryozoans and salmonid fish. Infected fish can develop Proliferative Kidney Disease, characterised by a gross lymphoid-driven kidney pathology in wild and farmed salmonids. To facilitate an in-depth understanding of T.bryosalmonae-host interactions, we have used a two-host parasite transcriptome sequencing approach in generating two parasite transcriptome assemblies; the first derived from parasite spore sacs isolated from infected bryozoans and the second from infected fish kidney tissues. This approach was adopted to minimize host contamination in the absence of a complete T.bryosalmonae genome. Parasite contigs common to both infected hosts (the intersect transcriptome; 7362 contigs) were typically AT-rich (60-75% AT). 5432 contigs within the intersect were annotated. 1930 unannotated contigs encoded for unknown transcripts. We have focused on transcripts encoding proteins involved in; nutrient acquisition, host-parasite interactions, development, cell-to-cell communication and proteins of unknown function, establishing their potential importance in each host by RT-qPCR. Host-specific expression profiles were evident, particularly in transcripts encoding proteases and proteins involved in lipid metabolism, cell adhesion, and development. We confirm for the first time the presence of homeobox proteins and a frizzled homologue in myxozoan parasites. The novel insights into myxozoan biology that this study reveals will help to focus research in developing future disease control strategies.