文献类型：期刊文献

英文题名：The impact of co-exposure to polystyrene microplastics and norethindrone on gill histology, antioxidant capacity, reproductive system, and gut microbiota in zebrafish ( Danio rerio )

作者：Zhou, Shuhui[1];Lin, Hongjie[1];Liu, Ziyun[3];Lian, Xiaoyi[3];Pan, Chang-Gui[5];Dong, Zhongdian[4];Lin, Zhong[3];Li, Chengyong[3];Hou, Liping[2];Liang, Yan-Qiu[3]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[2]Guangzhou Univ, Sch Life Sci, Guangzhou 510655, Peoples R China;[3]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[4]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[5]Guangxi Univ, Guangxi Lab Study Coral Reefs South China Sea, Nanning 530004, Peoples R China

年份：2024

卷号：273

外文期刊名：AQUATIC TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:001266432900001)、、Scopus(收录号：2-s2.0-85197383312)、WOS

基金：The authors would like to acknowledge the financial support from the Natural Science Foundation of Guangdong Province (2022A1515012107 and 2019A1515011889), Characteristic Innovation Projects of Ordinary Universities of Guangdong Province (2021KTSCX043) , National Natural Science Foundation of China (42177262, 41977207), and Scientific Research Fund for South China Sea Scholars Program of Guangdong Ocean University (002029002011) .

语种：英文

外文关键词：Microplastics; Progestin; Combined effects; Endocrine disrupting effects; Fish

外文摘要：In recent years, studies have focused on the combined ecological risks posed by microplastics and other organic pollutants. Although both microplastics and progestin residues are frequently detected in the aquatic environments, their ecological implications remain unknown. Adult zebrafish were exposed to polystyrene microplastics (PS, 200 nm, 200 mu g/L), norethindrone (NET, 69.6 ng/L), and their mixture (200 mu g/L PS + 63.1 ng/L NET) for 30 days. The results demonstrated that exposure to PS and NET resulted in gill damage. Notably, the PS and PS +NET exhibited a significant decrease in glutathione (GSH) and oxidized glutathione (GSSG) content, as well as reduced antioxidase activity in the gills. The oxidative stress in PS +NET primarily originated from PS. The PS, NET, or their mixture resulted in a decrease in testosterone (T) and estradiol (E2) levels in female. Furthermore, compared to NET, the PS +NET showed a significant reduction in E2 levels, thereby augmenting the inhibitory effect on reproductive ability mediated by NET. However, males showed an increase in 11-ketodihydrotestosterone (11-KT) content, accompanied by a significant decrease in spermatogonia (Sg) and increase in spermatocytes (Sc). Consequently, it can be inferred that PS enhances the androgenic effect of NET. In female fish brain, NET alone resulted in transcriptional down-regulation of partial hormone receptors; however, co-administration of PS effectively mitigated the interference effects. Furthermore, transcriptional downregulation of 17-alpha-hydroxylase ( cyp17 ), hydroxysteroid 3-beta dehydrogenase ( hsd3b ), estrogen receptor 1 ( esr1 ), and estrogen receptor 2a ( esr2b ) genes in the ovary was found to be associated with the androgenic activity induced by NET. Moreover, in comparison to PS or NET alone, PS +NET resulted in a notable decrease in Cetobacterium abundance and an increase in Aeromonas population, suggesting that the co-exposure of PS +NET may exacerbate intestinal burden. The findings highlight the importance of studying the combined toxicity of PS and NET.