文献类型：期刊文献

英文题名：Histological and transcriptional effects of androstenedione in adult zebrafish (Danio rerio)

作者：Liang, Yan-Qiu[1,2];Pan, Chang-Gui[3];Xie, Linchun[1];Huo, Jialiang[1];Yang, Jiahao[1];Lan, Tianhong[1];Dong, Zhongdian[4];Duan, Meina[1,2]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang, Peoples R China;[3]Guangxi Univ, Guangxi Lab Study Coral Reefs South China Sea, Nanning, Peoples R China;[4]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China

年份：2021

卷号：36

期号：4

起止页码：598

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000593032700001)、、EI(收录号：20204909563119)、Scopus(收录号：2-s2.0-85096819241)、WOS

基金：Guangdong Ocean University, Grant/Award Number: R17075; Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Grant/Award Number: ZJW-2019-08; Natural Science Foundation of Guangdong Province, Grant/Award Number: 2019A1515011889; National Natural Science Foundation of China, Grant/Award Numbers: 41806195, 41907346

语种：英文

外文关键词：androgen; androstenedione; histology; transcriptional effects; zebrafish

外文摘要：As a natural androgen, androstenedione (AED) may pose potential risks to aquatic organisms due to its ubiquitousness in aquatic environments. Here we assessed the adverse effects of AED on histology of gonads, as well as mRNA expression levels of 34 genes concerned with hypothalamic-pituitary-gonadal (HPG) axis, germ-cell differentiation and sex differentiation in zebrafish (Danio rerio). Adult zebrafish were exposed to solvent control and three measured concentrations of 0.2, 2.3 and 23.7 mu g/L AED for 60 days. The results showed that AED did not induce any obvious histological effects in the ovaries and testes. Of the investigated genes, transcriptional expression levels of amh and cyp11c1 genes in the ovaries of females were significantly increased by AED at 2.3 or 23.7 mu g/L. However, different exposure concentrations of AED significantly inhibited mRNA expression of gnrh3, atf4b1 and cyp19a1b in the brain of males. In the testes of males, AED at 2.3 mu g/L led to a significant induction of sox9b gene, but it at 23.7 mu g/L down-regulated nr5a1b gene. These observed transcriptional changes indicated that AED could pose potential androgenic effects in zebrafish.