文献类型：期刊文献

中文题名：鲍消化道及其养殖水体异养菌的耐药性研究

英文题名：Study of antibiotic-resistance of heterotrophic bacteria from intestines of abalone(Haliotis discus hannai) and farming water

作者：孙永婵[1,2];王瑞旋[2];赵曼曼[2];曹超[2];鲁义善[1];姚托[2];王江勇[2]

机构：[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室广东省水产经济动物病害控制重点实验室,广东湛江524088;[2]中国水产科学研究院南海水产研究所广东省渔业生态环境重点实验室农业部南海渔业资源开发利用重点实验室,广东广州510300

年份：2017

卷号：13

期号：3

起止页码：58

中文期刊名：南方水产科学

外文期刊名：South China Fisheries Science

收录：CSTPCD、、北大核心2014、CSCD2017_2018、北大核心、CSCD

基金：国家自然科学基金项目(41206118);广东省级鱼病防治专项资金(2014-585);广东省海洋渔业科技推广专项(A201301F04);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2014TS29;2016TS09)

语种：中文

中文关键词：皱纹盘鲍;异养细菌;抗菌药物;耐药率;多重耐药性

外文关键词：Haliotis discus hannai; heterotrophic bacteria; antimicrobial agents; resistance rate; multiple antibiotic resistance rate

中文摘要：采用纸片扩散法对分离自福建省皱纹盘鲍(Haliotis discus hannai)消化道及其养殖水体中的284株异养细菌(其中消化道菌131株,水体菌153株)进行药物敏感测试,以期了解其耐药性概况。结果显示,皱纹盘鲍消化道与养殖水体异养菌对多种抗菌药物的耐药率差异不明显,大部分菌株对青霉素、卡那霉素、庆大霉素、利福平产生耐药性,耐药率最高达83.95%,而对诺氟沙星、恩诺沙星、氯霉素耐药率较低,受试的水体异养菌对诺氟沙星耐药率为0;不同来源菌株的多重耐药(multiple antibiotic resistance,MAR)现象普遍,消化道及其养殖水体异养菌的多重耐药率均值分别为59.15%和51.31%。该研究同时发现相同分离源的菌株对青霉素、卡那霉素、庆大霉素和利福平的耐药率呈现一定的季节波动规律,消化道异养菌于9月耐药率最低,而水体异养菌耐药率在6月最低。结果表明,皱纹盘鲍消化道及水体异养菌的多重耐药率较高,耐药状况较严重。

外文摘要：A total of 284 strains （153 were isolated from intestines and 131 were isolated from farming water） were tested by Kirbybauer diffusion method to study the resistance of Haliotis discus hannai in Fujian Province. Results show that many strains resisted pen- icillin G, kanamycin, gentamicin and rifampin. The resistant rates were up to 83.59% ; the resistant rates to norfloxaein, chloram- phenieol and enrofloxaein were all relatively low, and resistant rate to norfloxacin was the lowest in farming water. Thus, there is no different resistance between heterotrophic bacteria from intestines and farming water. Multiple antibiotic resistance （MAR） was widespread in different sources and the average MARs were 59. 15% and 51.31% in intestines and farming water. The resistant rates to penicillin G, kanamycin, gentamicin and rifampin from intestines were the lowest in September and those from farming water were the lowest in June.