文献类型：期刊文献

英文题名：Methods for simulating nutritional requirement and response studies with all organisms to increase research efficiency

作者：Vedenov, Dmitry[1];Alhotan, Rashed A.[2];Wang, Runlian[2,3];Pesti, Gene M.[2]

机构：[1]Texas A&M Univ, Dept Agr Econ, College Stn, TX 77843 USA;[2]Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA;[3]Guangdong Ocean Univ, Dept Anim Sci, Zhanjiang 524088, Peoples R China

年份：2017

卷号：117

期号：3

起止页码：473

外文期刊名：BRITISH JOURNAL OF NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000398537800016)、、Scopus(收录号：2-s2.0-85013902023)、WOS

基金：This research was funded by Georgia Agricultural Experiment Station and Hatch project TEX0-1-9258.

语种：英文

外文关键词：Simulation; Nutritional requirements; Excel; Models

外文摘要：Nutritional requirements and responses of all organisms are estimated using various models representing the response to different dietary levels of the nutrient in question. To help nutritionists design experiments for estimating responses and requirements, we developed a simulation workbook using Microsoft Excel. The objective of the present study was to demonstrate the influence of different numbers of nutrient levels, ranges of nutrient levels and replications per nutrient level on the estimates of requirements based on common nutritional response models. The user provides estimates of the shape of the response curve, requirements and other parameters and observation to observation variation. The Excel workbook then produces 1-1000 randomly simulated responses based on the given response curve and estimates the standard errors of the requirement (and other parameters) from different models as an indication of the expected power of the experiment. Interpretations are based on the assumption that the smaller the standard error of the requirement, the more powerful the experiment. The user can see the potential effects of using one or more subjects, different nutrient levels, etc., on the expected outcome of future experiments. From a theoretical perspective, each organism should have some enzyme-catalysed reaction whose rate is limited by the availability of some limiting nutrient. The response to the limiting nutrient should therefore be similar to enzyme kinetics. In conclusion, the workbook eliminates some of the guesswork involved in designing experiments and determining the minimum number of subjects needed to achieve desired outcomes.