文献类型：期刊文献

中文题名：丛生盔形珊瑚共附生可培养真菌多样性分析

英文题名：Phylogenetic diversity analysis of cultured symbiotic fungi of Galaxea fascicularis L.

作者：徐佳[1];陈彬[2,3];雷晓凌[1];佘志刚[2];肖碧红[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524025;[2]中山大学化学与化学工程学院,广东广州510275;[3]西藏高原生物研究所,西藏拉萨850001

年份：2011

卷号：38

期号：8

起止页码：1193

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：广东海洋大学基金项目(No.E09095)

语种：中文

中文关键词：丛生盔形珊瑚;共附生;可培养;多样性;系统发育分析

外文关键词：Galaxeafascicularis, Fungal symbionts, Cultured, Diversity, Phylogenetic analysis

中文摘要：旨在研究珊瑚共附生可培养真菌多样性。运用稀释平板法和基于ITS-rDNA基因序列的系统发育分析对珊瑚共附生可培养真菌多样性进行研究,将所得到的基因序列与NCBI数据库GenBank中的序列进行相似性比较并构建系统发育树。实验中从丛生盔形珊瑚表面和内部共分离得到19株菌落形态各异的真菌。ITS-rDNA序列分析及形态学鉴定表明,丛生盔形珊瑚共附生真菌主要包括曲霉菌Aspergillus sp.、枝孢霉菌Cladosporium sp.、炭角菌Xylariales sp.、青霉菌Penicillium sp.、葡萄穗霉菌Stachybotrys sp.、赤霉菌Gibberella moniliformis、镰刀霉菌Fusarium sp.等。分离得到的菌株中,4-13与GenBank中已报道的基因序列的相似性仅为89%。结果表明,与丛生盔形珊瑚共附生的可培养真菌较为丰富,是潜在的新的微生物菌种资源,具有进一步研究的价值。

外文摘要：To study the diversity of cultured symbiotic fungi isolated from Galaxea fascicularis L. in the South China Sea. By dilution plate technique and ITS-rDNA sequence analysis to research the diversity of the cultured symbiotic fungi with the coral, determined sequences data were submitted to GenBank and compared with those known sequences. Phylogenetic tree were built up with MEGA 4.0 program. 19 different morphological strains were isolated from surface and inner of the coral Galaxea fascicularis. ITS-rDNA sequences analysis showed that, symbiosis cultured fungi mainly included Aspergillus sp., Cladosporium sp., Xylariales sp., Penicilliurn sp., Stachybotrys sp., Gibberella moniliformis, Fusarium sp. etc. The similarity of strain 4-13 sequence to the most closely related sequences in GenBank was only 89%. It is concluded that there are diverse fungal symbionts with coral Galaxea fascicularis, which have potential new microorganism resources, deserved for further study.