文献类型：期刊文献

英文题名：Polymorphisms in chicken extracellular fatty acid binding protein gene

作者：Zhao, Zhi-yuan[1];Zhang, Li[1];Du, Bing-wang[1];Cao, Ning-xian[2];Jiang, Xin-sheng[1];Tian, Wen-xia[2];Li, Shu-jun[2];Wu, Wei-wei[1];Ye, Chang-hui[1]

机构：[1]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China;[2]Livestock & Poultry Breeding Stn, Taiyuan 030001, Shanxi Province, Peoples R China

年份：2012

卷号：39

期号：3

起止页码：2677

外文期刊名：MOLECULAR BIOLOGY REPORTS

收录：SCI-EXPANDED(收录号：WOS:000301107800069)、、Scopus(收录号：2-s2.0-84863836186)、WOS

基金：This study was supported by the Program for Science and Technology Development of Guangdong Province (No. 2009B020415008), the Program for Science and Technology Development of Shanxi Province (No. 20100311042), the Program for Integration of Production, Teaching and Research of Guangdong Province (No. 2010B090400239), Guangdong Ocean University Doctor Seed Grant (No. 0712130), Guangdong Ocean University Natural Science Foundation (No. 1012129) and the Program for Science and Technology Development of Zhan Jiang city (No. 2010C3113002).

语种：英文

外文关键词：Extracellular fatty acid binding protein gene; Single nucleotide polymorphism; Chicken; Association analyses

外文摘要：In this study, we report the investigation of extracellular fatty acid binding protein gene (Ex-FABP) genetic polymorphism in a sample of 360 chicken individuals. The screening of the coding regions with their intron-exon boundaries and the proximal flanking regions was performed through a PCR-SSCP strategy. Following sequence analysis revealed 35 novel single nucleotide polymorphisms (SNPs) of chicken Ex-FABP gene. Among the 35 SNPs, twenty-five were found in the introns. And the remaining seven and three SNPs were in the coding region and the 5'UTR, respectively. Two SNPs in the coding region caused two missense mutants and the other five did not result in any amino acid changes. The nature and the distribution of Ex-FABP mutations in three chicken breeds were analyzed. Variations detected here might have an impact on Ex-FABP activity and function and underpin the development of gene markers for chicken fatty deposition and metabolism. The polymorphism, generated by C4715T mutation in exon5, was significantly associated with thickness of subcutaneous fat plus skin in cocks. Subcutaneous fat plus skin of cocks was more thick in TT genotype than in CC genotype (P < 0.05). The Ex-FABP gene could be a candidate locus or linked to a QTL that significantly affects fatty deposition and metabolism in chicken.