文献类型：期刊文献

中文题名：利用Fusarium poae制备T-2毒素的培养条件和提取方法

英文题名：Cultivation Condition and Extraction Method to Prepare T-2 Toxin Using Fusarium poae

作者：代喆[1];王雅玲[1];孙力军[1];施琦[1];徐德峰[1];王远征[2];胡红林[2];欧阳毅[2];吕国忠[2]

机构：[1]广东海洋大学食品科技学院,广东湛江524013;[2]大连民族学院环境与资源学院,辽宁大连116600

年份：2011

卷号：31

期号：5

起止页码：40

中文期刊名：微生物学杂志

外文期刊名：Journal of Microbiology

收录：CSTPCD、、CSCD_E2011_2012、CSCD

基金：国家自然科学基金(31171634);湛江市财政科技专项竞争性项目(2011D02)

语种：中文

中文关键词：T-2毒素;镰孢菌;产毒培养

外文关键词：T-2 toxin; Fusarium sp. ; toxin cultivation

中文摘要：比较2种不同优化方法对分离纯化梨孢镰孢菌(Fusarium poae)产生的T-2毒素的效果,并得到高纯度的T-2毒素,解决国内T-2毒素产业化问题。在优化Fusarium poae产毒培养条件的基础上,对Burmeister的提取方法和Gregory培养基进一步优化以最大限度地提高T-2毒素的产率,从而得到高纯度并且高产率的T-2毒素。目标菌株最适产毒培养基成分:NH4H2PO4 1 g,KCl 0.2 g,MgSO4.7H2O 0.2 g,葡萄糖10 g,酵母膏5g,CuSO4.5H2 O 0.005 g,ZnSO4.7H2 O 0.01 g,H2 O 1 000 mL。最佳产毒培养条件为8～25℃间隔12 h变温、前期光照后期黑暗、前期振荡后期静止的培养条件下培养28 d。在这种条件下可提高T-2毒素的产率达5倍。用丙酮氯仿进行分离,免疫亲和柱进行纯化得到高纯的T-2毒素。优化后的Burmeister提取方法提高了T-2毒素的产率。

外文摘要：The effects to isolate and purify T-2 toxin produced by Fusarium poae （FP） with two different optimized methods were compared and obtained high purity of T-2 toxin to solve its industrialization problem in China. Based on the optimization of toxin producing conditions by FP, Burmeister extraction and Gregory medium were further opti- mized in order to improve the productivity of T-2 toxin to the maximum thereby to obtain high purity and high productivity of T-2 toxin. The results showed that the most suitable toxin producing medium components of target strain were as follows： NH4H2PO4 1 g, KCl 0.2 g, MgSO4 - 7H20 0.2 g, glucose 10 g, yeast extract 5 g, CuSO4 · 5H2O 0. 005 g, ZnSO4 · 7H2O 0.01 g, H2O 1 000 mL. The most optimized conditions to produce toxin was euhured for 28 days at interval 8 - 25 ℃ of poikilothermia for 12 hours under illumination and shaking at early stage, then shading and culturing at rest at later stage, the production of T-2 toxin reached to maximal level of 5 mg, which was five times more than before. After separated by acetone-chloroform and purified by immune affinity column, and obtained high purity T-2 toxin. The optimized Burmeister extraction improved the productivity of T-2 toxin