文献类型：期刊文献

中文题名：超滤分离对虾头自溶产物ACE抑制活性的影响

英文题名：Effect of ultrafiltration on ACE inhibitory activity of autolysate of shrimp head

作者：朱国萍[1];章超桦[2,3];曹文红[2,3];吉宏武[2,3]

机构：[1]广东海洋大学分析测试中心;[2]广东海洋大学食品科技学院;[3]广东省水产品加工与安全重点实验室

年份：2013

卷号：22

期号：3

起止页码：452

中文期刊名：上海海洋大学学报

外文期刊名：Journal of Shanghai Ocean University

收录：CSTPCD、、北大核心2011、CSCD_E2013_2014、北大核心、CSCD

基金：国家科技支撑计划项目(2007BAD29B09);教育部科学技术重点项目(210155)

语种：中文

中文关键词：虾头;自溶产物;超滤;分子量分布;氨基酸;ACE抑制活性

外文关键词：shrimp head ; autolysate ; ultrafiltration ; distribution of relative molecular mass ; amino acid ; ACE inhibitory activity

中文摘要：采用3 000 u、5 000 u、8 000 u的超滤膜对凡纳滨对虾虾头自溶产物进行超滤分级分离,研究各组超滤组分的相对分子质量分布和氨基酸组成的变化,并比较各组超滤组分的血管紧张素转换酶(angiotensin-I-converting enzyme,ACE)抑制活性。结果表明:利用超滤膜分离虾头自溶液可提高虾头自溶产物的ACE抑制活性,3 000 u超滤组分的ACE抑制活性为0.61mg/mL,活性居各超滤组分之首;8 000 u超滤膜有效载留了大部分大分子物质,相对分子质量小于3 000 u的多肽随超滤孔径的减小而增多;3 000 u超滤组分中芳香族氨基酸和支链氨基酸均高于其余各超滤组分。

外文摘要：Litopenaeus vannamei head autolysate was consecutively extracted through ultrafiltration membrane with molecular weight cut-offs （MWCO） at 3 000, 5 000 and 8 000 u, respectively. The distribution of relative molecular mass, composition of amino acid as well as ACE （Angiotensin-I-converting enzyme ） inhibitory activity in different filtrates were measured. The results showed that ACE inhibitory activity of autolysate increased after extraction by extra-fine membrane. Obviously, the inhibitory activity of the filtrate of 3 000 u extra-fine membrane was 0. 61 mg/mL, which ranked the first compared with other filtrates. Meanwhile, the most of macromolecular substance was retained by 8 000 u while the extracted peptides with relative molecular mass less than 3 000 u increased markedly with the decline of MWCO of membrane. In addition, the contents of aromatic amino acid and branched chain amino acid in filtrate of 3 000 u were higher than those in other filtrates.