文献类型：期刊文献

英文题名：Preparation and evaluation of chitosan/alginate porous microspheres/Bletilla striata polysaccharide composite hemostatic sponges

作者：Wang, Chao[1,2];Luo, Wenfeng[1];Li, Puwang[2];Li, Sidong[3];Yang, Ziming[2];Hu, Zhang[3];Liu, Yangyang[2];Ao, Ningjian[1]

机构：[1]Jinan Univ, Inst Biomed Engn, Guangzhou 510632, Guangdong, Peoples R China;[2]Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China;[3]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China

年份：2017

卷号：174

起止页码：432

外文期刊名：CARBOHYDRATE POLYMERS

收录：SCI-EXPANDED(收录号：WOS:000407696800047)、、WOS

基金：This work was financially supported by the Natural Science Foundation of Guangdong Province (Nos. 2016A030307003; 2016A030308009), Project of Enhancing School With Innovation of Guangdong Ocean University (No. GDOU2013050221) and Central Public interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (1630122017009). We are also sincerely thankful to the teachers and students from the Agricultural Product Processing Research Institute at the Chinese Academy of Tropical Agricultural Sciences.

语种：英文

外文关键词：Chitosan; Alginate-calcium; Bletilla striata polysaccharide; Microspheres; Hemostatic sponge

外文摘要：We prepared a novel hemostatic material using chitosan (CS), alginate, and Bletilla striata, which showed good biocompatibility and hemostatic properties. Alginate-calcium porous microspheres (Alg) were prepared by emulsification/cross-linking methods, and Bletilla striata polysaccharide (Bsp) was extracted from the tubers by water extraction and alcohol precipitation. To improve the water absorption and accelerate the blood coagulation, Alg and Bsp were mixed with chitosan solution to form CS/Alg and CS/Alg/Bsp composite sponges by lyophilization. Based on SEM images, the microspheres exhibited good spherical shape, possessed many pores on the surface, and were well dispersed in the CS/Alg5 and CS/Alg10 composite sponges. The CS/Alg10 composite sponges showed higher water absorption and porosity. In addition, the cytotoxicity assay and hemostatic property of the CS/Alg and CS/Alg/Bsp composite sponges were tested by CCK-8 assay, in vitro blood clotting, red blood cell (RBC) adhesion, and rabbit ear artery bleeding. These ternary composite sponges could be used as potential novel hemostatic materials in surgical treatment. (C) 2017 Elsevier Ltd. All rights reserved.