文献类型：期刊文献

中文题名：吉富罗非鱼群体遗传多样性的AFLP分析

英文题名：Genetic Diversity of GIFT Tilapia Populations by AFLP Analysis

作者：燕涛[1];郭昱嵩[1];王中铎[1];刘丽[1];刘楚吾[1]

机构：[1]广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,湛江524025

年份：2013

卷号：32

期号：2

起止页码：207

中文期刊名：基因组学与应用生物学

外文期刊名：Genomics and Applied Biology

收录：CSTPCD、、北大核心2011、CSCD_E2013_2014、北大核心、CSCD

基金：广东省海洋渔业科技攻关与研发项目(A201008C03;A201101B02)资助

语种：中文

中文关键词：吉富罗非鱼;扩增片段长度多态性(AFLP);遗传多样性;多态位点比例

外文关键词：GIFT tilapia, AFLP, Genetic diversity, Proportion of polymorphic loci

中文摘要：为了分析吉富罗非鱼的遗传多样性,为其育种提供理论依据,本实验利用5对引物组合(E-AGG/M-CTT,E-AGG/M-CTG,E-AAC/M-CAG,E-ACA/M-CTG,E-ACA/M-CAG)对广东省化州光辉养殖场有限公司新选育的吉富罗非鱼第16代群体(F16)进行遗传多样性的AFLP分析。27个个体共检出187个扩增位点,其中多态位点163个,平均每对引物扩增出32.6个位点,多态位点比例87.17%;平均Nei's基因多样性0.2886;平均Shannon's指数0.4339。结果说明实验群体存在较丰富的遗传多样性且保持有较高的遗传杂合度,具有进一步育种和开发的价值。

外文摘要：The experiment was conducted to assess the genetic diversity of genetically improved farmed tilapia （GIFT） in order to evaluate and preserve this strain as a germplasm resource. The experiment study focused on the genetic diversity of the newly bred F 16 population of GIFT tilapia obtained from Guanghui Farm Limited Company, Huazhou （Guangdong, China） was analyzed using AFLP. 187 bands were detected with 5 pairs of AFLP primers based on 27 individuals, and among them 163 （87.17%） were polymorphic with an average of 32.6 bands for per AFLP primer. Average Nei＇s gene diversity was 0.288 6, average Shannon＇s information index was 0.433 9. These results showed that the experiment group deposit had rich genetic diversity and maintained a high genetic heterozygosity, had further breeding and development value.