文献类型：期刊文献

中文题名：低鱼粉饲料添加丁酸梭菌对凡纳滨对虾生长、抗氧化能力和非特异性免疫的影响

英文题名：Effects of Clostridium butyricum on Growth,Antioxidant Capacity and Non-specific Immunology of Litopenaeus vannamei Fed with Concentrated Cottonseed Protein Replacement of Fishmeal

作者：李维康[1,2,3];李立贤[1,2,3];刘泓宇[1,2,3];谭北平[1,2,3];董晓慧[1,2,3];杨奇慧[1,2,3];迟淑艳[1,2,3];章双[1,2,3];谢瑞涛[4]

机构：[1]广东海洋大学水产学院;[2]农业部华南水产与畜禽饲料重点实验室;[3]广东省水产动物精准营养与高效饲料工程技术研究中心;[4]广东恒兴饲料实业股份有限公司,广东湛江524088

年份：2022

卷号：42

期号：2

起止页码：29

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、北大核心、北大核心2020

基金：国家重点研发计划(2019YFD0900200);国家自然科学基金(31772864);广东省自然科学基金(2018A030313154,2020A1515011129);科技厅企业特派员项目(GDKTP2020017800)。

语种：中文

中文关键词：浓缩棉籽蛋白;丁酸梭菌;凡纳滨对虾;生长;非特异性免疫;抗氧化能力

外文关键词：concentrated cottonseed protein;Clostridium butyricum;Litopenaeus vannamei;growth performance;non-specific immunity;antioxidant capacity

中文摘要：【目的】研究浓缩棉籽蛋白替代鱼粉饲料中添加丁酸梭菌(Clostridium butyricum)对凡纳滨对虾(Litopenaeus vannamei)生长性能、血清生化、抗氧化能力、免疫力和肠道消化能力的影响。【方法】取(0.87±0.06)g的健康凡纳滨对虾,随机分为6组,分别投喂鱼粉组(PC)、替代组(NC,浓缩棉籽蛋白替代30%鱼粉蛋白)、替代组基础上添加质量分数0.03%(C1,3×10^(8) cfu/kg)、0.12%(C2,1.2×10^(9) cfu/kg)、0.48%(C3,4.8×10^(9) cfu/kg)和1.92%(C4,1.92×10^(10) cfu/kg)丁酸梭菌的实验饲料8周,测定对虾生长性能、肠道消化酶活性、抗氧化能力及非特异性免疫指标。【结果】PC组和C2组对虾增重率(WGR)和特定生长率(SGR)显著高于C4组(P<0.05),饲料系数(FCR)显著低于C4组(P<0.05)。PC组血清甘油三酯(TG)、总胆固醇(T-CHO)、低密度脂蛋白(LDL)和酸性磷酸酶(ACP)显著高于NC组(P<0.05),谷丙转氨酶(GPT)显著低于NC组(P<0.05)。相比于PC组与NC组,各实验组血清TG、T-CHO、LDL、高密度脂蛋白(HDL)含量均显著降低(P<0.05),C2组碱性磷酸酶(AKP)显著升高(P<0.05),C3组ACP显著升高(P<0.05),C4组血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)、溶菌酶(LMZ)、淀粉酶(AMS)、胰蛋白酶(TRY)和脂肪酶(LIP)显著升高(P<0.05),丙二醛(MDA)含量和谷草转氨酶(GOT)显著降低(P<0.05)。【结论】在本研究条件下,当浓缩棉籽蛋白替代30%鱼粉蛋白时,饲料中添加丁酸梭菌可显著提高凡纳滨对虾抗氧化能力、肠道消化能力和非特异性免疫力。以WGR和SGR为判据,丁酸梭菌的最适添加量为1.2×10^(9) cfu/kg。

外文摘要：【Objective】Clostridium butyricum and its metabolites,including butyric acid,have been shown to repair intestinal damage,maintain intestinal micro-ecological balance and improve immunity.This experiment was conducted to investigate the effects of C.butyricum on growth performance,serum biochemical indexes,antioxidant capacity,immunity and intestinal digestive enzymes of Litopenaeus vannamei fed with concentrated cottonseed protein replacement fishmeal.【Method】Healthy L.vannamei[(0.87±0.06)g]were randomly divided into 6 groups.They were fed with either fish meal(PC),alternative basic feed with concentrated cottonseed protein replaced 30%fish meal protein(NC),C.butyricum supplemented with 0.03%(C1,3×10^(8) cfu/kg),0.12%(C2,1.2×10^(9) cfu/kg),0.48%(C3,4.8×10^(9) cfu/kg),and 1.92%(C4,1.92×10^(10) cfu/kg)of alternative group basic feed,respectively.The experiment lasted for 8 weeks.【Result】The shrimp weight gain rate(WGR)and specific growth rate(SGR)were significantly higher in the PC and C2 groups than those in the C4 group(P<0.05),and the feed conversion ratio(FCR)was significantly lower compared to the C4 group(P<0.05).Serum triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein(LDL)and acid phosphatase(ACP)were significantly higher in the PC group than in the NC group(P<0.05);alanine aminotransferase(GPT)were significantly lower than in the NC group(P<0.05).Compared with the PC and NC groups,serum TG,T-CHO,LDL and high density lipoprotein(HDL)were significantly lower in all experimental groups(P<0.05);alkaline phosphatase(AKP)were significantly higher in C2 group(P<0.05);ACP were significantly higher in C3 group(P<0.05);serum total antioxidant capacity(T-AOC),superoxide dismutase(SOD),glutathione peroxidase(GPX),lysozyme(LMZ),amylase(AMS),trypsin(TRY)and lipase(LIP)were significantly higher and malondialdehyde(MDA)and aspartate aminotransferase(GOT)were significantly lower in C4 group(P<0.05).【Conclusion】Diet supplementation with C.butyricum significantly improved the antioxidant capacity,intestinal digestibility and non-specific immunity of L.vannamei when cottonseed protein concentrate replaced 30%fishmeal protein.The most optimal supplementation level for C.butyricum was 1.2×10^(9) cfu/kg,using WGR and SGR as assessment criteria.