文献类型：期刊文献

中文题名：来自普哥滨珊瑚的一株Talaromyces sp.真菌的活性次生代谢产物

英文题名：Bioactive secondary metabolites from the fungus Talaromyces sp. isolated from coral Porites pukoensis

作者：聂影影[1,2];刘亚月[2];杨文聪[2];黎燕媚[2];许茂鑫[2];雷晓凌[2];张翼[1,2]

机构：[1]广东海洋大学深圳研究院,广东深圳518000;[2]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东省海洋食品工程技术研究中心水产品深加工广东普通高等学校重点实验室湛江市脑健康海洋药物与营养品重点实验室广东海洋大学海洋药物研究所

年份：2019

卷号：38

期号：4

起止页码：585

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：广东省扬帆计划引进紧缺拔尖人才项目(201433009);广东省自然科学基金(2018A030307046);广东海洋大学科研启动项目(E15155);深圳市科创委基础研究自由探索项目(JCYJ20170306165013264);广东省应用科技研发重大专项(2016B020235001)~~

语种：中文

中文关键词：内生真菌;(R)-(-)-hydroxysydonic;acid;homodimeric;WIN;64821;抗菌活性;乙酰胆碱酯酶抑制活性

外文关键词：endophytic fungi;(R)-(-)-hydroxysydonic acid;homodimeric WIN 64821;antimicrobial activity;acetylcholinesterase inhibitory activity

中文摘要：利用硅胶柱层析、制备型HPLC和重结晶等手段从普哥滨珊瑚分离的一株Talaromycessp.真菌C21-1中筛选得到2个活性化合物,运用核磁共振、质谱和圆二色谱等技术鉴定这两个化合物分别为(R)-(-)-hydroxysydonic acid(1)和homodimeric WIN 64821(2),补充完善了化合物2的核磁共振信号归属,并对化合物进行抗菌活性和乙酰胆碱酯酶抑制活性的测定,发现化合物1对白色假丝酵母Canidia albicans和耐甲氧基青霉素的金黄色葡萄球菌Methicillin-resistantStaphylococcusaureus(MRSA)具有一定的抑制作用,其最低抑菌浓度(MIC)分别为0.075mmol/L和0.2mmol/L,对副溶血弧菌Vibrio parahemolyticus的抑制活性较弱,在0.2mmol/L浓度下的抑制率为17%;化合物2最大浓度0.2mmol/L条件下对这3种菌均没有明显的抑制效果。化合物2表现出剂量依赖的乙酰胆碱酯酶抑制活性,0.5mmol/L时抑制率达到35.1%。

外文摘要：The fungus Talaromyces sp.C21-1 was isolated from the coral Porites pukoensis collected in Xuwen of Guangdong Province.Two bioactive compounds were obtained by silica gel column chromatography,preparation HPLC and recrystallization and their structures were identified as(R)-(-)-hydroxysydonic acid(1)and homodimeric WIN 64821(2)by NMR,MS and CD methods.The assignment of the NMR data for compound 2 was achieved by detailed analysis of 2 D NMR data and comparison with previous reports.Their biological activities were investigated including antimicrobial activities and acetylcholinesterase inhibitory activity.The compound 1 showed moderate inhibitory activities to Canidia albicans and methicillin-resistant Staphylococcus aureus(MRSA)with the minimum inhibitory concentrations(MIC)at 0.075 mmol/L and 0.2 mmol/L respectively,and exhibited weak inhibitory activity against Vibrio parahemolyticus with inhibition rate of 17%at the concentration of 0.2 mmol/L.The antimicrobial activities of compound 2 against the above mentioned three microorganisms were not significant under the concentration of 0.2 mmol/L.Compound 2 also showed dose dependent inhibition to acetylcholinesterase reached the inhibition rate of 35.1%at0.5 mmol/L.