文献类型：期刊文献

英文题名：Customized Screening of Ace2 Regulatory Peptides from Oyster to Mitigate Lps-Induced Acute Lung Injury Based on Spr Technology

作者：Xia, Xiaoyu[1,2]; Wen, Caixing[1]; Cao, Wenhong[1,2]; Qin, Xiaoming[1,2]; Li, Yujin[3]; Lin, Haisheng[1,2]; Chen, Zhongqin[1,2]; Zheng, Huina[1,2]; Zhu, Guoping[1,2]; Gao, Jialong[1,2]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; [2] National Research and Development Branch Center for Shellfish Processing [Zhanjiang], Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [3] College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

年份：2025

外文期刊名：SSRN

收录：EI(收录号：20250092662)

语种：英文

外文关键词：Biological organs - Cell death - Diagnosis - Diseases - Lung cancer - Molluscs - Shellfish - Surface plasmon resonance - Tissue

外文摘要：Oyster protein hydrolysate (OPH) exhibits various healthful activities. We found OPH ameliorates acute lung injury (ALI) of mice, however, the effective components remain to be explored. This study aims to screen peptides targeting angiotensin converting enzyme 2 (ACE2) in OPH for ALI treatment by surface plasmon resonance (SPR) and ?liquid chromatograph-mass spectrometer (LC-MS/MS). Since OPH promoted ACE2 expression in lung tissue, five peptides P1-P5 from OPH were screened via ACE2-immobilized SPR chip (immobilization level of 14221.9 RU) and identified by LC-MS/MS. Among them, P5 performed a "slow rise/slow fall" binding form with ACE2, showing a long-term binding effect. P3 and P5 effectively alleviated oxidative stress in LPS-induced A549 cells. Moreover, P5 (KSVSPKFLTG) reduced inflammation and pyroptosis in lung tissue of ALI mice via promoting ACE2 expression. This customized screening based on SPR technology provides a theoretical basis for efficient and accurate screening of active factors from food sources. ? 2025, The Authors. All rights reserved.