文献类型：期刊文献

英文题名：Optimized preparation of gastric acid-response sulfhydryl functionalized chitosan/alginate/tilapia peptide hydrogel and its protective effects on alcohol-induced liver and brain injury

作者：Lu, Sitong[1];Zhang, Lingyu[1];Hu, Zhang[1];Kong, Songzhi[1];Zhang, Zhaoyu[1];Li, Guangfa[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm Sci, Dept Appl Chem, Zhanjiang 524088, Peoples R China

年份：2021

卷号：11

期号：55

起止页码：34544

外文期刊名：RSC ADVANCES

收录：SCI-EXPANDED(收录号：WOS:000711069100001)、、EI(收录号：20214811233786)、Scopus(收录号：2-s2.0-85119720224)、WOS

基金：This work was funded by Guangdong Province Natural Science Foundation of China (2016A030308009) National Key Research and Development Program of China (2020YFD0901101), Project of Science and Technology Plan of Zhanjiang (2019A01017 and 2020A01026), Guangdong Ocean University Undergraduate Innovation Team Project (CXTD2021020) and Graduate Education Innovation Program of Guangdong Ocean University (201926).

语种：英文

外文关键词：Ascorbic acid - Peptides - Diseases - Antioxidants - Mammals - Sodium alginate - Ethanol - Hydrogels - Amino acids - Swelling

外文摘要：Long-term alcohol intake or drinking large quantities of alcohol at one time can cause organ damage, which in turn can lead to chronic diseases. It is of important clinical and social significance to find effective approaches for the prevention and treatment of alcohol-induced diseases. In this paper, sulfhydryl functionalized chitosan (chitosan-N-acetyl-l-cysteine, CS-NAC) and sodium alginate (SA) were used as the matrix materials to contain tilapia peptide (TP), and a gastric acid-response hydrogel (CS-NAC/SA/TP) was prepared. Taking the ethanol adsorption rate as the response index, based on the results of the single factor test, the preparation process of CS-NAC/SA/TP was optimized through the Box-Behnken design. The swelling and antioxidant properties of CS-NAC/SA/TP were tested in vitro, and the protective effects on alcohol-induced acute liver injury and chronic brain injury were assessed in vivo. Structural characterization showed that CS-NAC/SA/TP was successfully prepared. Under the optimal conditions (SA concentration of 1%, MCS-NAC/MCaCO3 of 1 : 1, MSA/MCS-NAC(CaCO3) of 15 : 1), the prepared CS-NAC/SA/TP had a porous structure, a swelling ratio of 2350%, an ethanol adsorption rate of 56.23% and strong antioxidant capacities in vitro. Animal experiment results demonstrated that CS-NAC/SA/TP effectively reduced liver and brain injuries in mice caused by alcoholism. Summarily, these findings indicate that CS-NAC/SA/TP has potential applications in preventing alcohol-induced liver and brain injuries.