文献类型：期刊文献

中文题名：菲律宾蛤仔糖胺聚糖RG-1的结构特征及免疫活性研究

英文题名：Structural Characteristics and Immunological Activity of Glycosaminoglycan RG-1 from Ruditapes Philippinarum

作者：范秀萍[1];王瑞芳[1];吴红棉[1];王娅楠[1];胡雪琼[1];雷晓凌[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524006

年份：2012

卷号：12

期号：2

起止页码：186

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD、、北大核心2011、Scopus、CSCD_E2011_2012、北大核心、CSCD

语种：中文

中文关键词：菲律宾蛤仔;糖胺聚糖;结构分析;免疫调节

外文关键词：Ruditapes philippinarum ; glycosaminoglycan（RG-1 ） ; structural analysis ; immunoregulation

中文摘要：目的:对菲律宾蛤仔糖胺聚糖RG-1的一级结构特征及细胞免疫调节活性进行研究。方法:采用高效液相色谱法测定相对分子质量和单糖组成,通过红外光谱、高碘酸氧化和Smith降解等研究其糖链主要结构特征;用MTT法分析对小鼠脾淋巴细胞的影响。结果:RG-1主要由葡萄糖、氨基半乳糖和半乳糖通过1→6、1→4或1→4,6键连接构成主链,在主链中还含有大量的半乳糖醛酸,重均相对分子质量为9.94×105。RG-1具有显著增强小鼠腹腔巨噬细胞吞噬中性红的作用和脾淋巴细胞的增殖作用(p<0.01)。结论:菲律宾蛤仔糖胺聚糖可通过增强细胞免疫活性而起到抗肿瘤作用。

外文摘要：Objective： To investigate the structural characterstics and cells immunomodulatory activity of the glycosaminoglycan RG-1 from Ruditapes philippinarum. Methods： The morlicular weight and monosaccharide composition of the glycosaminoglycan RG-1 was analysed by HPGPC, its main structure characterstics was studied by IR, periodate oxidation and Smith degradation. Effects of RG-1 on proliferation of KM mouse spleen lymphocytes were detected by MTT. Results： RG-1 was composed of Glc-GalNAc-Gal by 1→6,1→4 or 1→4,6 in main chain backbone which contained much galacturonic acid with Mr 9.94×105. RG-1 significantly simulated the phagocytic activity of mice peritoneal macrophages and proliferation of KM mouse spleen lymphocytes in vitro （p〈0.01）. Conclusion：N Glycosaminoglycan RG-1 can inhibit the cancer by enhance cell immunological activity.