文献类型：期刊文献

中文题名：中国毛虾酶解产物的分子量分布及ACE抑制活性

英文题名：Angiotensin I-converting enzyme inhibitory activity and molecular weight distribution of peptic hydrolysate of Acetes chinensis

作者：曹文红[1];秦小明[1];章超桦[1];王长云[2]

机构：[1]广东海洋大学食品科技学院;[2]中国海洋大学海洋药物教育部重点实验室,青岛266003

年份：2007

卷号：32

期号：5

起止页码：145

中文期刊名：食品科技

外文期刊名：Food Science and Technology

收录：北大核心2004、北大核心

基金：中国海洋大学教育部海洋药物重点实验室开放基金资助项目[KLMD(OUC)2004]

语种：中文

中文关键词：中国毛虾;酶解;ACE抑制活性;分子量分布

外文关键词：Acetes chinensis; enzymatic hydrolysis; angiotensin Ⅰ-converting enzyme inhibition activity; molecular weight distribution

中文摘要：利用胃蛋白酶酶解中国毛虾制备具有血管紧张素转化酶抑制活性的酶解产物,并采用凝胶色谱法探讨酶解产物的分子量分布。结果表明:在45℃、pH2.5、酶量2%(w/w)、酶解4h、底物浓度20%(w/v)条件下,中国毛虾酶解产物的ACE抑制率高达85.9%;Sephadex G-15葡聚糖凝胶色谱图上出现5个吸收峰,其中高ACE抑制活性组分的分子量在1320～311之间,IC50为0.77mg/mL。

外文摘要：Pepsin was employed to hydrolyze Acetes chinensis to prepare hydrolysate with angiotensin Ⅰ-converting enzyme inhibitory activity. Gel column filtration chromatography was used to determine the molecular distribution of the hydrolysate. The results showed that inhibition ratio of the hydrolysate was up to 85.9%, which was prepared under the condition of 45℃, pH2.5, enzyme concentration 2%（w/w） and substrate concentration 20%（w/v）. Gel filtration of the hydrolysate on Sephadex G-15 chromatogram yielded five absorbance peaks, the molecular distribution of the fractions with the most potent inhibition activity was located between 1320 and 311, the IC50 was 0.77mg/mL.