文献类型：期刊文献

英文题名：Heterotrophic assimilation and simultaneous nitrification-denitrification drive nitrogen cycling in a commercial biofloc system for Penaeus vannamei production

作者：Luo, Zhishen[1,2];Liu, Hongyu[1];Cao, Yucheng[1,2];Zhao, Yongzhen[3];Zhang, Bin[3];Su, Haochang[2];Hu, Xiaojuan[2];Xu, Yu[2];Xu, Wujie[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Southern Marine Sci & Engn Guangdong Lab, Guangzhou 510300, Peoples R China;[3]Guangxi Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Aquaculture Genet & Breeding & Hlth Aquacu, Key Lab Comprehens Dev & Utilizat Aquat Germplasm, Nanning 530021, Peoples R China

年份：2027

卷号：627

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001863365300001)、、WOS

基金：This research was funded by National Natural Science Foundation of China (NSFC 32373135) , Guangxi Science and Technology Major Program (AA23062046, AA23062047) , Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (SML2024SP003) , the Earmarked Fund for CARS-47, Innovative Team Building Project of Guangdong Modern Agricultural Industrial Technology System (2024CXTD25) , and Central Public-interest Scientific Institution Basal Research Fund, CAFS (2023TD57) .

语种：英文

外文关键词：Biofloc technology; Nitrogen cycling; Nitrification-denitrification; Functional redundancy; P. vannamei

外文摘要：Efficient nitrogen (N) management is a key determinant of production stability and environmental performance in commercial biofloc technology (BFT) systems for shrimp aquaculture. Here, we elucidated microbial N-cycling mechanisms by integrating N mass balance with metagenomics in a commercial-scale (30 m(3)) BFT system with Penaeus vannamei production for 56 days. The system achieved robust production (7.87 kg m(-3) yield, 94.3% survival) and 39.6% feed N utilization efficiency. The N balance was characterized by two main nitrogen sinks: heterotrophic assimilation converted 15.0% of input N into harvestable bioflocs, while an apparent nitrogen loss of 26.6% was observed, likely driven by simultaneous nitrification-denitrification (SND). Microbial analysis revealed that despite significant temporal taxonomic turnover (ANOSIM, r = 0.889), the abundance of core Ncycling genes remained highly stable, indicating functional redundancy that buffers N-removal against community shifts. Nitrosomonas was identified as a key bacterial group associated with a highly diverse functional gene repertoire. Under the evaluated C/N ratio of 12, a balance between autotrophic and heterotrophic bacteria was observed, facilitating efficient SND and biofloc production. Consequently, this process theoretically promotes alkalinity recovery and reduces pH buffering costs. This research provides a mechanistic and practical basis for scaling up biofloc technology in sustainable, resource-efficient intensive Penaeus vannamei production.