文献类型：期刊文献

中文题名：鲍鱼肌肉酶解产物对秀丽隐杆线虫的抗衰老作用研究

英文题名：Anti-aging effect of Abalone muscle enzymatic hydrolysate on Caenorhabditis elegans

作者：蔡佳琦[1];秦小明[1,2];卢虹玉[1,2];叶琬祺[1];张桂芳[1];林海生[1,2];郑惠娜[1,2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东湛江524088;[2]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

年份：2023

卷号：49

期号：12

起止页码：106

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、CSCD2023_2024、北大核心、CSCD、北大核心2020

基金：财政部和农业农村部国家现代农业产业技术体系资助(CARS-49)。

语种：中文

中文关键词：鲍鱼;酶解产物;秀丽隐杆线虫;抗衰老;氧化应激

外文关键词：Abalone;enzymatic hydrolysate;Caenorhabditis elegans;anti-aging;oxidative stress

中文摘要：该研究以鲍鱼为原料,制备鲍鱼肌肉酶解产物(Abalone muscle water extract residue enzymatic product,ARE)并进行成分分析。通过对秀丽隐杆线虫寿命、运动能力、体内脂褐素水平、繁殖能力、抗应激能力等的分析测定,评价ARE抗衰老活性。结果表明,ARE主要成分为蛋白质;与对照组相比,高中低剂量的ARE均能显著延长秀丽隐杆线虫的寿命、提高线虫的运动能力(A型运动比例增加)、降低体内脂褐素水平,其中最高剂量ARE处理组(2.50 mg/mL)的作用最强,上述指标水平分别改变了17.36%、56.66%和21.93%(P<0.05)。ARE能够提高线虫的抗应激能力,与对照组相比,2.50 mg/mL ARE处理组能够提高线虫热应激损伤存活率35.85%,增加了百草枯氧化应激损伤线虫的平均寿命25.73%。ARE提高了线虫体内抗氧化酶超氧化物岐化酶和谷胱甘肽的水平,显著降低了线虫体内丙二醛含量。另外,ARE在延长寿命的同时提高了繁殖能力。这些结果表明ARE具有较好的抗衰老活性,为开发鲍鱼功能性食品奠定了良好的基础。

外文摘要：In present study,the Abalone muscle water extract residue enzymatic product(ARE)was prepared and analyzed.To evaluate the anti-aging activity of ARE,longevity,exercise ability,lipofuscin level,reproductive ability,and anti-stress ability of Caenorhabditis elegans were detected respectively.The results showed that the main component of ARE was protein.Compared with the control group,the high,middle,and low dose of ARE could significantly prolong the life span of C.elegans,improve the movement ability of C.elegans(the proportion of type A movement increased),and reduce the level of lipofuscin in vivo.Among them,the highest dose of ARE treatment group(2.50 mg/mL)had the strongest effect.The levels of the above indicators changed by 17.36%,56.66%,and 21.93%respectively(P<0.05).ARE improved the anti-stress ability of C.elegans.Specifically,the 2.5 mg/mL ARE treatment group compared with the control group improved the survival rate of heat-damaged C.elegans by 35.85%,and increased the average life span of C.elegans by 25.73%under paraquat induced stress condition.Meanwhile,ARE increased the levels of superoxide dismutase(SOD)and glutathione(GSH)in C.elegans and significantly reduced malondialdehyde(MDA)level.In addition,ARE increased reproductive capacity accompanied with prolonged life span of C.elegans.In conclusion,ARE exhibits good anti-aging activity,which lays a good foundation for the development of abalone functional food.