文献类型：期刊文献

中文题名：对虾肠道中产毒镰孢菌抑制菌的筛选与鉴定

英文题名：Isolation and Identification of Strains Inhibiting Toxigenic Fusarium Isolated from Intestinal Bacteria of Prawns

作者：叶日英[1];王雅玲[1];孙力军[1];徐德峰[1];蔡坤荣[1];薜奕红[1];陈妍贤[1];刘昆[1]

机构：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室

年份：2015

卷号：0

期号：7

起止页码：117

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD、、北大核心2014、EI(收录号：20153501225798)、Scopus(收录号：2-s2.0-84940395909)、北大核心

基金：国家自然科学基金资助项目(31171634;31371777;31371746);"十二五"国家科技支撑计划项目(2012BAD29B06);广东海洋大学大学生创新创业训练项目(CXXL2014024);湛江市科技计划项目(2011D02);广东海洋大学创新强校工程科研项目(GDOU2013050205;GDOU2014050203)

语种：中文

中文关键词：对虾肠道细菌;禾谷镰孢菌;T-2毒素;抑制

外文关键词：prawn intestinal bacteria;;Fusarium graminearum;;T-2 toxin;;inhibition

中文摘要：为了获得对产毒镰孢菌具有抑制效应的海洋细菌,从对虾肠道中分离细菌,并将分离得到的优势细菌与产T-2毒素的禾谷镰孢菌(Fusarium graminearum)(FG1207)进行固体对峙培养,具有抑菌圈的细菌菌落即为产毒镰孢菌的抑制菌。然后选取对FG1207具有抑制作用的细菌与FG1207进行液体共同培养,用LC-MS/MS技术检测菌悬液中T-2毒素含量,最后对具有抑制FG1207的生长和降解T-2毒素效果的细菌进行16s r RNA序列鉴定和VITEK2细菌生化鉴定。实验结果分离得到8株优势细菌,其中一株对FG1207的生长具有明显的抑制作用;LC-MS/MS检测发现该细菌与FG1207共同培养菌悬液中未检测到T-2毒素,说明该细菌不仅能够抑制产毒镰孢菌的生长,还能降解T-2毒素。经16s r RNA鉴定该细菌为海洋尼泊尔葡萄球菌(Nepal Staphylococcus Aureus),相似度为99.93%,VITEK2细菌生化鉴定的相似度为96.86%。

外文摘要：In order to identify marine bacteria with the ability to inhibit the toxigenic fungus Fusarium, beneficial bacteria were isolated from prawns intestines and cultured on a solid medium in antagonism with Fusarium graminearum(FG1207), which produces the T-2 toxin. Bacteria with inhibition zones were regarded as being able to inhibit toxigenic FG1207. These were isolated and grown with FG1207 in a liquid medium. Liquid chromatography-mass spectroscopy/ mass spectroscopy(LC-MS/MS) was used to estimate the T-2 toxin present in the liquid medium. Bacterial strains found to both inhibit FG1207 growth and degrade T-2 toxin were identified using 16 s r RNA sequencing and VITEK 2 biochemical identification methods. Thus, eight strains of beneficial bacteria were isolated. Of these, one strain(H5) showed a significant inhibitory effect on FG1207 growth, wherein LC-MS/MS did not detect T-2 toxin in the liquid medium containing H5 and FG1207. This result indicated that not only could this bacterial strain inhibit the growth of toxigenic Fusarium but it could also degrade T-2 toxin. The strain H5 was identified via 16 s r RNA sequencing as Nepal Staphylococcus aureus, with 99.93% similarity seen, while VITEK 2 biochemical identification showed 96.86% similarity.