文献类型：期刊文献

英文题名：Antibacterial Mechanism of Slightly Acidic Electrolyzed Water Against Pseudomonas Arsenicoxydans A1 from Greater Amberjack (Seriola Dumerili)

作者：Zhong, Hongliang[1]; Liu, Yinglin[1]; Wei, Shuai[1,2]; Khan, Imran[3]; Sun, Qinxiu[1]; Wang, Zefu[1]; Han, Zongyuan[1]; Liu, Yang[1]; Oh, Deog-Hwan[4]; Liu, Shucheng[1,2]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [2] Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China; [3] Department of Food Science and Technology, The University of Haripur, Haripur, 22620, Pakistan; [4] Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, 24341, Korea, Republic of

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230359299)

语种：英文

外文关键词：Bacteria - Cell death - Enzyme activity - Fish - Spoilage

外文摘要：This study aimed to identify the specific spoilage organism (SSO) from the metamorphosed greater amberjack (Seriola dumerili) fish. P. arsenicoxydans A1 was identified as SSO by culture-depending method. Further, the antibacterial efficacy of slightly acidic electrolyzed water (SAEW) was explored and the mechanism of action on P. arsenicoxydans A1 was studied. SAEW has shown good antibacterial effect on P. arsenicoxydans A1. The total number of colonies, growth curve, OD value at 260 nm and 280 nm, DNA fluorescence intensity, AKP, LDH, CAT, ATP enzyme activity and SEM results of inoculated fish fillets and pure bacterial solution showed that SAEW could destroy the permeability and integrity of P. arsenicoxydans A1 cell membrane and cell wall, and affect bacterial cell activity at metabolic, enzymatic and DNA level, which caused bacterial cell death. This study provided the application of SAEW in sterilization and preservation of raw fish fillets, which showed a promising effect on the growth of P. arsenicoxydans A1 in food cold chain. ? 2023, The Authors. All rights reserved.