文献类型：期刊文献

英文题名：Iturin A Strongly Inhibits the Growth and T-2 Toxin Synthesis of Fusarium oxysporum: A Morphological, Cellular, and Transcriptomics Study

作者：Hua, Meifang[1];Deng, Qi[1];Qiu, Mei[1];Deng, Yijia[2];Sun, Lijun[1];Fang, Zhijia[1];Liao, Jianmeng[3];Zhao, Jian[4];Gooneratne, Ravi[5]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol,Guangdong Prov Engn Lab M, Guangdong Prov Engn Technol Res Ctr Seafood,Guang, Zhanjiang 525088, Peoples R China;[2]Southwest Univ, Coll Food Sci, Chongqing 500715, Peoples R China;[3]Zhanjiang Inst Food & Drug Control, Zhanjiang 525022, Peoples R China;[4]Univ New South Wales, Sch Chem Engn, Sydney, NSW 2052, Australia;[5]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, POB 85085, Lincoln 7657, New Zealand

年份：2023

卷号：12

期号：6

外文期刊名：FOODS

收录：SCI-EXPANDED(收录号：WOS:000955593000001)、、WOS

语种：英文

外文关键词：iturin A; Fusarium oxysporum; reactive oxygen species; T-2 toxin; Tri5

外文摘要：Fusarium oxysporum (F. oxysporum) is a common contaminant of dried fish, and the T-2 synthesis by this organism in dried fish products poses a serious public health risk. In this study, we investigated the effects of iturin A, a cyclic lipopeptide produced by Bacillus subtilis, on the growth and synthesis of the T-2 toxin of F. oxysporum, and transcriptomics was conducted. Results showed that the inhibitory effect of iturin A on F. oxysporum was significantly enhanced with an increase in iturin A concentrations. More specifically, compared with the control group, all indexes in the iturin A treatment group with 50 mu g/mL were decreased to 24.84 mm, 0.33 x 10(6) cfu/mL, and 5.86 ng/mL for the colony diameter, number of spores, and concentration of T-2 toxin, respectively. Furthermore, iturin A was proven to destroy the integrity of cell membranes and cause a significant increase in ROS at 25 mu g/mL or 50 mu g/mL. Transcriptomic analysis revealed that with the treatment of iturin A, the genes of the oxidation-reduction process were up-regulated, while the gene expression of mycelial growth, cell integrity, transmembrane transport, energy metabolism, and others were down-regulated. More importantly, the Tri5 gene cluster was significantly inhibited. This study provided new insights into the mechanism for the inhibitory effect of iturin A on the growth and T-2 toxin synthesis of F. oxysporum and theoretical guidance for the application of iturin A in the preservation of dried aquatic products.