文献类型：期刊文献

英文题名：Impact of polyvinyl chloride microplastic exposure on the hepatopancreas metabolism of Pinctada fucata martensii: Insights from metabolomics

作者：Lu, Fenglan[1];Li, Zhisheng[1];Yang, Chuangye[1,2,3,4,5];Liao, Yongshan[2];Mkuye, Robert[1];Deng, Yuewen[1,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China;[3]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[4]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[6]Guangdong Marine Ecol Early Warning & Monitoring L, Zhanjiang 524088, Peoples R China

年份：2024

卷号：304

外文期刊名：ESTUARINE COASTAL AND SHELF SCIENCE

收录：SCI-EXPANDED(收录号：WOS:001257365100001)、、Scopus(收录号：2-s2.0-85195033096)、WOS

基金：This work was supported by the Science and Technology Program of Guangdong Province (Grant No. 2023A1515011769) , National Natural Science Foundation of China (Grant No. 32102817) , Technology Program of Guangdong Zhanjiang (Grant No. 2022A01010) , Department of Education of Guangdong Province (Grant No. 2020ZDZX1045 and 2021KCXTD026) , the earmarked fund for CARS-49, Special Fund for Guangdong Province's Science and Technology Innovation Strategy (Grant No. pdjh2024a191) , the program for scientific research start-up funds of Guangdong Ocean University (060302022304 and 060302022305) , and Hengli Biosciences Excellence Project of Guang-dong Ocean University (Grant No. B23335-4) . We are very grateful to Marine Pearl Science and Technology Backyard in Leizhou of Guang-dong for collecting samples. The authors would like to thank TopEdit ( www.topeditsci.com ) for its linguistic assistance during the preparation of this manuscript. Metabolomics analysis was assisted by Biotree Biotech Co., Ltd. (Shanghai, China) .

语种：英文

外文关键词：Microplastics; Pinctada fucata martensii; Hepatopancreas; Metabolomics

外文摘要：Microplastics (MPs) are prevalent throughout the oceans and pose a significant global environmental concern due to their detrimental effects on various living organisms. While researchers have investigated the toxicity mechanisms of MPs in marine species, there is a need for a more comprehensive understanding, particularly regarding the potential adverse effects on the growth and metabolism of bivalves. In this study, indoor exposure experiments were conducted on Pinctada fucata martensii to examine the impact of commercial polyvinyl chloride (PVC)-MPs (50 mu m, 15 mg/L). The aim was to determine the effects of MPs on the hepatopancreas metabolism of P. f. martensii. Non-targeted metabolomics analysis indicated alterations in the hepatopancreatic metabolic of P. f. martensii following exposure to PVC-MPs. A total of 32, 27, and 154 differential metabolites (SDM) were identified in EG (1-d) vs CG (1-d), EG (15-d) vs CG (15-d), and EG (1-d) vs EG (15-d), respectively (VIP >1 and P < 0.05). Further examination of metabolic pathways revealed that PVC-MPs disrupted the metabolism of amino acids, particularly alanine, aspartic acid, and glutamic acid metabolism; this suggests that the organism may have developed neurotoxicity. Sphingolipid metabolites showed a significant up-regulation in both EG (15-d) vs CG (15-d), as well as in EG (1-d) vs EG (15-d), indicating lipid peroxidation in the hepatopancreas of P. f. martensii following PVC-MPs exposure. Additionally, in EG (1-d) vs CG (1-d), starch and sucrose metabolism were significantly inhibited, glycolysis or gluconeogenesis pathways were promoted, and changes in energy metabolism were promoted. This study sheds light on the impact of MPs on the metabolism of bivalves, offering fresh insights into the detrimental effects of MPs on aquatic organisms.