文献类型：期刊文献

中文题名：低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响

英文题名：Hypoxia stress on growth, serum biochemical and non-specific immune indexes of juvenile cobia (Rachycentron canadum)

作者：王维政[1];曾泽乾[1];黄建盛[1,2,3];郭志雄[1];李洪娟[1];陈刚[1,2,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524025;[3]广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088

年份：2021

卷号：43

期号：2

起止页码：49

中文期刊名：海洋学报

外文期刊名：Acta Oceanologica Sinica

收录：CSTPCD、、CSCD2021_2022、Scopus、北大核心、CSCD、北大核心2020

基金：现代农业产业技术体系专项资金(CARS-47);南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2019-06);广东海洋大学大学生创新创业训练计划项目(CXXL2018131)。

语种：中文

中文关键词：军曹鱼;低氧;生长;生化指标;非特异性免疫

外文关键词：Rachycentron canadum;hypoxia;growth;biochemical indexes;non-specific immune

中文摘要：为了研究低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响,将体重为(50.44±2.78)g的军曹鱼幼鱼在(3.15±0.21)mg/L的低氧环境下养殖4周,分别在第1天、第7天、第14天、第28天取样并进行相关指标的测定。结果表明,在低氧胁迫过程中:(1)军曹鱼幼鱼生长受到低氧的抑制,增重率和特定生长率显著低于对照水平(p<0.05)。(2)血清肝功能指标表现为不同程度的上升趋势,其中谷草转氨酶(AST)在第7天、第14天、第28天极显著高于对照组(p<0.01);谷丙转氨酶活性在第14天、第28天极显著高于对照组(p<0.01);总蛋白和白蛋白含量均在第14天和第28天与对照组有显著性差异(p<0.05)。(3)血清糖脂水平表现为波动升高的趋势,其中血糖含量在第1天、第14天和第28天与对照水平有显著性差异(p<0.05);甘油三酯含量在第14天和第28天极显著高于对照水平(p<0.01);总胆固醇含量在第1天显著下降(p<0.05)后呈不断上升的趋势。(4)血清离子含量变化情况各异,其中钠离子和氯离子含量在第14天和第28天极显著升高(p<0.01);钾离子含量不断升高并在第7天、第14天和第28天与对照组具有显著性差异(p<0.05);钙离子含量则呈不断下降的变化趋势,在第14天和第28天显著低于对照水平(p<0.05)。(5)血清超氧化物歧化酶活性持续升高,并在第7天、第14天、第28天极显著高于对照组(p<0.01);丙二醛含量持续上升,并在第14天、第28天极显著高于对照组(p<0.01);过氧化氢酶活性表现为“下降?上升?下降”变化趋势;溶菌酶活性在第1天显著升高(p<0.05)后下降。研究结果显示,低氧胁迫对军曹鱼幼鱼造成一定程度的氧化损伤,抑制其生长并使血清生化和非特异性免疫相关指标发生显著变化,表明军曹鱼机体物质代谢和免疫系统受到影响。

外文摘要：In order to study the effect of hypoxia stress on serum of biochemical and non-specific immune indexes of the juvenile cobia(Rachycentron canadum),the fish with body weight of(50.44±2.78)g were treated at dissolved oxygen content of(3.15±0.21)mg/L for 4 weeks.The samples were taken on 1 d,7 d,14 d and 28 d respectively for determination of relevant indexes and results showed that:(1)the growth of juvenile cobia was inhibited by hypoxia,the weight gain rate and specific growth rate were significantly lower than the control level(p<0.05);(2)the indexes of liver function showed that activities of aspartate aminotransferase and alanine aminotransferase significantly increased(p<0.01)on 7 d,14 d,28 d and 14 d,28 d respectively,while the content of total protein and albumin significantly increased(p<0.05)on 14 d and 28 d than that of the control group;(3)the content of blood glucose significantly increased(p<0.05)on 1 d,14 d and 28 d,while the triglyceride significantly increased(p<0.01)on 14 d and 28 d.The total cholesterol content significantly decreased(p<0.05)on 1 d,then continue to increase;(4)the serum ion content changed with different trend,in which sodium ion and chloride ion significantly increased(p<0.01)on 14 d and 28 d.Potassium content increased continuously and was significantly higher(p<0.05)on 7 d,14 d and 28 d than control level,while calcium content continued to decrease and there was a significant difference(p<0.05)on 14 d and 28 d;(5)activitie of superoxide dismutase and content of malondialdehyde continued to increase and were significantly higher(p<0.01)than those in the control group on 7 d,14 d,28 d and 14 d,28 d respectively.Activities of catalase showed significantly“decreased-increased-decreased”patterns.Activitie of lysozyme significantly increased(p<0.05)on 1 d and then continued to decrease.These results showed that hypoxia stress caused oxidative damage to the juvenile cobia,inhibited its growth and led to significant change in serum biochemical and non-specific immune indexes,which indicating that the nutrient utilization and immune system of cobia were affected.