文献类型：期刊文献

英文题名：Differential expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in orange-spotted grouper (Epinephelus coioides)

作者：Hou, Qing-Hua[1,2,3];Yi, Shi-Bai[1,2];Ding, Xu[1,2,4];Zhang, Hui-Xian[1,2];Sun, Yan[1,2];Zhang, Yong[1,2];Liu, Xiao-Chun[1,2];Lu, Dan-Qi[1,2];Lin, Hao-Ran[1,2]

机构：[1]Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Ocean & Meteorol, Zhanjiang, Guangdong, Peoples R China;[4]Hainan Univ, Ocean Coll, Haikou, Hainan, Peoples R China

年份：2012

卷号：33

期号：5

起止页码：1102

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000311191700005)、、WOS

基金：This project was supported by the National 863 Program of China (No. 2012AA10A407 and 2012AA092201), the Special Fund for Agro-scientific Research in the Public Interest (No. 200903046), Jiangsu Provincial Science and Technology Achievements Program Special Fund (No.BA2009083), the National Natural Science Foundation of China (Grant No. 31001126), the Fundamental Research Funds for the Central Universities (Grant No. 11lgpy64), Specialized Research Fund for the Doctoral Program of Higher Education (Grant No.20110171130005), the Guangdong Provincial Science and Technology Program (No. 2010B090400551, 2011B020307009), and the Guangdong Natural Science Foundation (Grant No.10451027501005658).

语种：英文

外文关键词：Orange-spotted grouper; NOD1; NOD2; RICK; IL-8

外文摘要：Nucleotide-binding oligomerization domain-containing proteins-1 and -2 (NOD1 and NOD2) are members of the NOD-like receptors (NLRs) family. They are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. In this study, the full-length ORF sequences of NOD1 and NOD2 were cloned, and the putative amino acid sequences were identified in orange-spotted grouper (Epinephelus coioides). The complete open reading frame (ORF) of grouper NOD1 contained 2823 bp encoding a 940 amino acid protein. Grouper NOD2 cDNA contained a 2967 bp ORF, encoding a protein of 988 amino acid residues. Both grouper NOD1 and NOD2 had similar domains to human and fish counterparts. Phylogenetic tree analysis showed that grouper NOD1 clustered with grass carp, zebrafish and channel catfish, while NOD2 was most closely related to fugu. Expression patterns of grouper NOD1 and NOD2 were next studied. NOD1 had the highest level of expression in skin while NOD2 in trunk kidney. Post Vibrio alginolyticus (strain EcGS020401), lipopolysaccharide (LPS) or PolyI:C challenges, gene expression of grouper NOD1 and NOD2 was stimulated to different extents. NOD1 showed a significant enhancement after LPS stimulation, but NOD2 increased more significantly after PolyI:C invasion, indicating that NOD1 and NOD2 may exert different effects on the eradication of bacteria and virus. The adaptor protein RIP-like-interacting CLARP kinase (RICK) and downstream molecule interleukin-8 (IL-8) were also induced at different levels after stimulation, which indicated that NOD1 and NOD2 signal transduction was involved in grouper innate immune protection against bacterial and viral infections. (C) 2012 Elsevier Ltd. All rights reserved.