文献类型：期刊文献

中文题名：对虾中T-2毒素的残留规律及其对雄性小鼠的遗传毒性效应

英文题名：Patterns of T-2 Toxin Residue in Prawns and Its Subsequent Effect on Genes in Male Mice

作者：吴朝金[1];莫冰[2];王雅玲[1];黄展锐[1];何婉莹[1];苏静怡[1];徐德峰[1];孙力军[1]

机构：[1]广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,广东海洋大学食品科技学院;[2]广西壮族自治区产品质量监督检验研究院

年份：2015

卷号：31

期号：2

起止页码：1

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD、、北大核心2014、EI(收录号：20151700790154)、Scopus(收录号：2-s2.0-84928237371)、北大核心

基金：国家自然科学基金资助项目(31171634;31371777)

语种：中文

中文关键词：对虾;T-2毒素;雄性小鼠;精子畸形;微核

外文关键词：prawn; T-2 toxin; male mice; sperm deformity; micronucleus

中文摘要：研究了对虾中T-2毒素急性暴露的残留规律,并探明其对雄性小鼠的遗传毒性效应。实验以LC-MS/MS检测肌肉注射染毒后,对虾中T-2毒素残留量,并经灌胃,检测毒虾中T-2毒素对小鼠遗传毒性的危害。结果发现,对虾中游离态T-2毒素的残留量与暴露剂量呈正相关,表现蓄积性,并且不同组织的蓄积量差异较大,以肌肉中含量最低,接近于零。经毒虾灌胃后,小鼠的精子畸形率及微核率也随暴露剂量逐渐增大。与对照组相比,高剂量灌胃组的小鼠精子畸形率和微核率出现显著性变化(P<0.05),高剂量组的四个对虾组织都具有毒性,其中以对虾肌肉和肝胰腺的毒性最强,其次为肠道。表明T-2毒素在对虾体内具有隐蔽性,特别是在肌肉中,游离态T-2毒素易于被转化为隐蔽态T-2毒素,从逃避检测,并产生二次危害隐患。

外文摘要：This study examined the pattern of T-2 toxin residue in prawns following acute exposure, and the genetic alterations in mice following administration of the poisoned prawns. Liquid chromatography-tandem mass spectrometry（LC-MS/MS） was used to detect the content of residual T-2 toxin in prawns after intramuscular injection. The mice were administered the poisoned prawns by gavage. The results showed that the content of free T-2 toxin in prawns positively correlated with its increasing dose. T-2 toxin appeared to have accumulated in the prawns, and the amounts were significantly different among the various organs. The lowest content of free T-2 toxin was found in muscle; T-2 toxin content was close to zero. The sperm deformity and micronucleus rate of mice gradually increased with an increase in the exposure dose, compared to the control group（P〈0.05）. The four organs of the prawns in the high dose group were toxic to the mice, and the toxicity levels were the highest in the muscles and hepatopancreas, followed by the intestinal tract. The results indicated that T-2 toxin in prawns could be easily concealed, especially in the muscle, where free T-2 toxin is converted to masked T-2 toxin. As a result, T-2 toxin could remain undetected, resulting in secondary harm to other organisms.