文献类型：期刊文献

英文题名：Molecular signatures of in vitro produced embryos derived from ovum pick up or slaughterhouse oocytes in buffalo

作者：Yang, Chun-Yan[1,2];Zheng, Hai-Ying[1];Abdelnour, Sameh A.[1,2,3];Li, Ling-yu[1];Shokrollahi, Borhan[1,4];Tang, Li-Ping[1];Zhang, Yu[1,5];Huang, Jia-Xiang[1];Shang, Jiang-Hua[1]

机构：[1]Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Key Lab Buffalo Genet Breeding & Reprod Technol, Nanning 530001, Peoples R China;[2]Guangxi Univ, Coll Anim Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China;[3]Zagazig Univ, Fac Agr, Anim Prod Dept, Zagazig 44511, Egypt;[4]Islamic Azad Univ, Fac Agr, Dept Anim Sci, Sanandaj Branch, Sanandaj, Iran;[5]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China

年份：2021

卷号：169

起止页码：14

外文期刊名：THERIOGENOLOGY

收录：SCI-EXPANDED(收录号：WOS:000649286200003)、、Scopus(收录号：2-s2.0-85104599835)、WOS

基金：This work was supported by grants from the Guangxi Natural Science Foundation (2018GXNSFAA050129, 2020 GXNSFAA159002), the National Key Research and Development Program of China (2017YFE0113800), the Key Research and Development Program in Guangxi (GuiKeAB1850013) and Guangxi Agricultural Science and Technology Project (Z201951&Z201967).

语种：英文

外文关键词：Buffalo; Abattoir ovaries; Ovum pick up; Development competence; Molecular mechanism

外文摘要：This study was performed to investigate the difference in developmental competence of oocytes derived from ovum pick-up (OPU) and slaughterhouse ovaries (SLH), and its underlying mechanisms. The OPU and SLH oocytes were in-vitro maturated and fertilized to produce blastocysts, and these blastoycsts were collected to explore the expression of key genes for developmental potential and telomere (Oct-4, Sox2, Nanog, Cdx2, Gata3, E-cadherin, b-catenin, TERT, TERF1 and TERF2). The results showed that both the cleavage and blastocyst rates were significantly higher for the OPU group (68.31%, 39.48%, respectively) than SLH group (57.59%, 26.50%, respectively) (P < 0.01). The relative mRNA abundances of Sox2, Oct-4, Nanog and E-cadherin were significantly higher in the OPU blastocysts than the SLH ones (P < 0.01). Protein expression analysis by Western blot and immunofluorescence also revealed that the expression of E-cadherin and Sox2 was significantly higher in OPU blastocysts than SLH ones. However, there was no significant differences between the two groups in the expression of Cdx2, b-catenin, Gata3, TERT, TERF1, TERF2. These results imply oocyte sources modify the expression of development and adhesion related genes in blastocysts, which may elucidate a possible reasoning for the low development competence of buffalo SLH embryos. (c) 2021 Elsevier Inc. All rights reserved.