文献类型：期刊文献

英文题名：A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues

作者：Deng, Qi[1];Qiu, Mei[1,3];Wang, Yaling[1];Lv, Pengli[1];Wu, Chaojin[1];Sun, Lijun[1];Ye, Riying[1];Xu, Defeng[1];Liu, Ying[1];Gooneratne, Ravi[2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Lincoln Univ, Ctr Food Res & Innovat, POB 85084, Lincoln 7647, New Zealand;[3]Natl Marine Prod Qual Supervis & Inspect Ctr, Zhanjiang 524096, Peoples R China

年份：2017

卷号：142

起止页码：441

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：SCI-EXPANDED(收录号：WOS:000403384300053)、、Scopus(收录号：2-s2.0-85018357366)、WOS

基金：This study was financially supported by the National Natural Science Foundation of China (NSFC) (No. 31171634 and No. 31371777), and Science and Technology Planning Project of Guangdong Province (No.2014B020205006), and Guangdong oceanic & fishery administration project (Guangdong Fiscal Government on Agriculture [2015] No. 115), and the Project of Enhancing School with Innovation of Guangdong Ocean University (No.2013050205 and No.2014050203 and GDOU2013050312).

语种：英文

外文关键词：T-2 toxins; Immunomagnetic; ELISA; Shrimp tissues

外文摘要：Accurate analyses of total T-2 (free and modified) in aquatic organisms including shrimp are important as the hazard caused by T-2 has been caught increasing attention. Therefore, acurate analysis of free T-2 especially of modified T-2 in shrimp tissues is important. A rapid, sensitive, and validated method for quantitative determination of free T-2 and modified T-2 toxin was developed using immunomagnetic-bead based enzyme linked immunosorbent assay (IMB-ELISA). Super paramagnetic particles with a carboxyl group activated by an ester method coupled with envelope antigen 3- acetylneosolaniol- hemisuccinate - ovalbumin (3-Ac-NEOS-HS-OVA) was used to form immunomagnetic beads which could bind to T-2 skeletal structure antibodies. The conditions for magnetic bead coating of T-2 skeletal structure antibodies, and the concentrations of the polyclonal antibody and HRP-labeled goat anti-rabbit antibody were optimized. A good linear relationship with T-2 concentrations ranging from 5 75 ng/mL (R-2 = 0.9965) was observed. The detection limit of different shrimp tissues of the IMB-ELISA ranged from 2.53 to 3.20 ng/mL. And the IC50 was 63 ng/mL. The recovery varied from 86% to 99% with a standard deviation of 2.8-5.8%. The application of this method to study the distribution in tissues showed that the total T-2 concentration in hepatopancreas was 26.7 mu/kg > blood > head > muscle in the highest dose group of 12.2 mg/kg. Our research showed a combination of ELISA and immunomagnetic bead technology provide a new, convenient approach to significantly improve the accuracy and sensitivity of total T-2 measurement in shrimp tissues.