文献类型：期刊文献

英文题名：A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection

作者：Li, Guoliang[1,2];Tang, Chenhong[3,4];Wang, Ying[2];Yang, Jing[2];Wu, Hongliang[5];Chen, Guang[2];Kong, Xiaojian[2];Kong, Weiheng[2];Liu, Shucheng[1];You, Jinmao[2,5]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Product Proc & Safet, Zhanjiang 524000, Peoples R China;[2]Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 27365, Peoples R China;[3]Arizona State Univ, Biodesign Inst, Ctr Bioenerget, Tempe, AZ 85287 USA;[4]Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA;[5]Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China

年份：2015

卷号：8

期号：7

起止页码：1804

外文期刊名：FOOD ANALYTICAL METHODS

收录：SCI-EXPANDED(收录号：WOS:000358326700021)、、EI(收录号：20153101099703)、Scopus(收录号：2-s2.0-84938259544)、WOS

基金：This work was supported by The National Natural Science Foundation of China (No. 31301595, 21475075, 21475074, and 21275089), the Open Project Program of Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Ocean University (GDPKLAPPS1401), PhD research start-up funds of Qufu Normal University (bsqd 2012017), and the Soft Science Project of Shandong Province (2013RKA08020).

语种：英文

外文关键词：Semicarbazide; HPLC; Fluorescence labeling; Foodstuffs

外文摘要：Semicarbazide (SEM) has been proven to extensively exist in foodstuffs due to anthropogenic factor in food processing and possesses various toxic effects on human health. Although many methods have been developed, they often require long analytical time, complex laboratory equipment, trained personnel, difficultly prepared antibodies, or relatively expensive equipment. The present study developed a new method for SEM determination by HPLC with fluorescence detection (FLD). The fluorescence reagent, 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC), was first used for SEM labeling. The fluorescent labeling conditions were optimized systematically. SEM can be labeled in only 10 min at 40 A degrees C. The labeled SEM was analyzed on an eclipse XDB-C8 column in 8 min. The new method offered the low LOD of 0.4 mu g/kg at a signal-to-noise ratio of 3 and also exhibited excellent reproducibility, precision, and accuracy. When applied to analyze several foodstuffs, it showed good applicability. The developed method has been proven to be simple, inexpensive, selective, sensitive, accurate, and reliable for SEM analysis in foodstuffs. Furthermore, this developed method should have a powerful potential in the analysis of SEM from many other food samples.