文献类型：期刊文献

中文题名：纤维素分解酶对凤梨渣青贮品质及干物质降解率的影响

英文题名：Effects of Cellulase on the Quality and Dry Matter Degradation Rate of Pineapple Residue Silage

作者：王志敬[1,2];吴征敏[1];尹福泉[1];谭伟军[2];高振华[1];赵志辉[1]

机构：[1]广东海洋大学滨海农业学院,广东湛江524000;[2]广东省清远市动物疫病预防控制中心,广东清远511518

年份：2022

卷号：44

期号：6

起止页码：1399

中文期刊名：江西农业大学学报

外文期刊名：Acta Agriculturae Universitatis Jiangxiensis

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家自然科学基金项目(31772562)。

语种：中文

中文关键词：凤梨渣;青贮品质;干物质降解率;相对饲喂价值;纤维素分解酶

外文关键词：pineapple residue;silage quality;dry matter degradation rate;RFV;cellulase

中文摘要：【目的】探究纤维素分解酶对凤梨渣青贮品质及干物质降解率的影响,为凤梨渣的资源化利用提供参考。【方法】选择新鲜凤梨渣,风干至含水量为65%~70%后,将纤维素分解酶按鲜重以0,1500,3000,6000 u/kg添加量添加至凤梨渣中(分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组),混合均匀后装入青贮容器中,每组18个重复,置于通风、避光、干燥处,常温青贮4周后测定常规养分指标和干物质降解率,干物质降解率经体外瘤胃液培养后,通过失重法测定。【结果】添加纤维素分解酶可提高粗蛋白(CP)(P<0.05)、乳酸(LA)(P<0.05)、乙酸(AA)、丙酸(PA)、总挥发性脂肪酸(TVFA)(P<0.05)含量以及乳酸与总挥发性脂肪酸比值(LA/TVFA)(P<0.05)和相对饲用价值(RFV),降低氨态氮(NH3-N)(P<0.05)、粗纤维(CF)、中性洗涤纤维(NDF)(P<0.05)、酸性洗涤纤维(ADF)含量以及氨态氮与总氮比值(NH3-N/TN)(P<0.05)和pH;Ⅱ、Ⅲ、Ⅳ组的CP分别较对照组提高13.86%、19.45%、13.58%;Ⅱ、Ⅲ、Ⅳ组的LA分别较对照组提高21.79%、20.10%、33.83%;Ⅱ、Ⅲ、Ⅳ组的NH3-N和NH3-N/TN分别较对照组降低11.69%、29.87%、24.68%和22.54%、41.97%、33.81%;Ⅱ、Ⅲ、Ⅳ组的RFV较对照组分别提高2.35%、2.85%、3.52%;Ⅱ组亚硝酸盐含量和pH最低。方差分析结果显示,纤维素分解酶添加水平和发酵时间对干物质降解率(DMD)的影响均达到极显著水平(P<0.01);纤维素分解酶添加水平和体外发酵时间与干物质降解率均呈极显著正相关关系(P<0.01);纤维素分解酶添加组干物质降解率均高于对照组;CP、LA、AA、PA、TVFA、LA/TVFA、RFV等指标与DMD呈正相关关系(P<0.05),NH3-N、NH3-N/TN、NDF等指标与DMD呈负相关关系(P<0.05)。【结论】纤维素分解酶可以提高凤梨渣青贮品质和干物质降解率,且以1500~3000 u/kg添加量为宜。

外文摘要：[Objective]This study aims to explore the effect of cellulase on the quality of pineapple residue silage and the degradation rate of dry matter,thus providing a reference for the resource utilization of pineapple residue.[Method]Cellulase were added to pineapple residue with the addition of 0 u/kg,1 500 U/kg,3 000 u/kg and 6 000 u/kg(groups Ⅰ,Ⅱ,Ⅲ and Ⅳ),with 3 repetitions in each group.Samples were taken after silage at room temperature for 4 weeks to determine the fermentation quality and dry matter degradation rate.[Result]The results showed that adding cellulase could increase CP(P<0.05),LA(P<0.05),AA,PA,TVFA(P<0.05),LA/TVFA(P<0.05)and RFV;the adding could decrease NH_(3)-N(P<0.05),NH_(3)-N/TVFA(P<0.05),CF,CF,pH(P<0.05),ADF,NDF,pH;CP and WSC contents in Group Ⅱ,Ⅲ were the highest,NH_(3)-N,NH_(3)-N/TN in Group Ⅲ were the lowest;nitrite contents and pH in Group Ⅱ were the lowest.The results of variance analysis showed that the addition level of cellulase and fermentation time were significantly related to DMD(P<0.01),the addition level of cellulase and Rumen fermentation time were positively correlated with the dry matter degradation rate(P<0.01),and the dry matter degradation rate of the cellulase-added group was higher than that of the Control Group.Based on the results of the experiment and variance analysis,it can be concluded that cellulase can regulate the DMD of pineapple residue silage by increasing CP,LA,AA,PA,TV-FA,LA/TVFA,RFV,and decreasing NH_(3)-N,NH_(3)-N/TN,NDF.[Conclusion]In conclusion,cellulase can improve the silage quality and dry matter degradation rate of pineapple residue,and the appropriate addition amount is 1 500-3 000 u/kg.