文献类型：期刊文献

英文题名：Effects of Heat Stress on Production Performance, Redox Status, Intestinal Morphology and Barrier-Related Gene Expression, Cecal Microbiome, and Metabolome in Indigenous Broiler Chickens

作者：Liu, Wen-Chao[1];Pan, Zi-Yi[1];Zhao, Yue[1];Guo, Yan[1];Qiu, Sheng-Jian[1];Balasubramanian, Balamuralikrishnan[2];Jha, Rajesh[3]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Anim Sci, Zhanjiang, Peoples R China;[2]Sejong Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Seoul, South Korea;[3]Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Human Nutr Food & Anim Sci, Honolulu, HI USA

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PHYSIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000795299600001)、、Scopus(收录号：2-s2.0-85129783869)、WOS

基金：Funding This research was funded by the National Nature Science Foundation of China (32002196).

语种：英文

外文关键词：antioxidant capacity; cecal microbiota; cecal metabolome; heat stress; intestinal barrier function; slow-growing broilers

外文摘要：This study was done to evaluate the effects of heat stress (HS) on production performance, redox status, small intestinal barrier-related parameters, cecal microbiota, and metabolome of indigenous broilers. A total of forty female indigenous broilers (56-day-old) were randomly and equally divided into normal treatment group (NT group, 21.3 +/- 1.2 degrees C, 24 h/day) and HS group (32.5 +/- 1.4 degrees C, 8 h/day) with five replicates of each for 4 weeks feeding trial. The results showed that the body weight gain (BWG) of broilers in HS group was lower than those in NT group during 3-4 weeks and 1-4 weeks (p < 0.05). The HS exposure increased the abdominal fat rate (p < 0.05) but decreased the thigh muscle rate (p < 0.01). Besides, broilers in HS group had higher drip loss of breast muscle than NT group (p < 0.01). Broilers exposed to HS had lower total antioxidant capacity (T-AOC) in serum and jejunum, activities of total superoxide dismutase (T-SOD) in the jejunum, glutathione peroxidase (GSH-Px) in the thigh muscle, duodenum, and jejunum; and catalase (CAT) in breast muscle, duodenum, and jejunum (p < 0.05). Whereas the malondialdehyde (MDA) contents in breast muscle, duodenum, and jejunum was elevated by HS exposure (p < 0.05). Moreover, the relative mRNA expression of Occludin and ZO-1 in the duodenum, Occludin, Claudin-1, Claudin-4, ZO-1, Mucin-2 in the jejunum, and the Claudin-4 and Mucin-2 in the ileum was down-regulated by HS exposure (p < 0.05). The 16S rRNA sequencing results showed that the HS group increased the relative abundance of Anaerovorax in the cecum at the genus level (p < 0.05). Cecal metabolomics analysis indicated 19 differential metabolites between the two groups (p < 0.10, VIP >1). The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the differential metabolites mainly enriched in 10 signaling pathways such as the Citrate cycle (TCA cycle) (p < 0.01). In summary, chronic HS exposure caused a decline of production performance, reduced antioxidant capacity, disrupted intestinal barrier function, and negatively affected cecal microbiota and metabolome in indigenous broilers.