文献类型：期刊文献

中文题名：无瓣海桑根响应盐胁迫的转录组分析

英文题名：Transcriptome Analysis of Sonneratia apetala Root in Response to Salt Stress

作者：梁锐涛[1];韩维栋[1];杨少瑕[1];陈蓓蓓[1]

机构：[1]广东海洋大学滨海农业学院,广东湛江524088

年份：2023

卷号：36

期号：1

起止页码：68

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD、、Scopus、CSCD2023_2024、北大核心、CSCD、北大核心2020

基金：国家自然科学基金项目(31901330);湛江市科技发展专项资金竞争性分配项目(2020A01007);广东海洋大学科研启动费资助项目(R19047)。

语种：中文

中文关键词：无瓣海桑;盐胁迫;转录组;差异表达基因

外文关键词：Sonneratia apetala;salt stress;transcriptome;differentially expressed genes(DEGs)

中文摘要：[目的]初步探究无瓣海桑的耐盐分子机制,筛选出无瓣海桑抗盐候选基因,为后续功能验证实验及林木抗盐性遗传育种奠定分子基础。[方法]以1年生无瓣海桑幼苗为材料,用500 mmol·L^(-1)NaCl分别处理0 d(对照组)和10 d(处理组),取不同条件下的根部组织进行转录组测序,并结合三代全长转录组数据进行后续生物信息学分析。[结果](1)与对照组相比,NaCl处理10 d后,无瓣海桑幼苗根系中共有14 401个差异表达基因,其中,7 153个上调,7 248个下调。(2)GO分析发现,共有11 068个差异基因在47个GO条目得到注释。(3)在KEGG富集分析中,共有6 189个差异基因富集到134条通路,其中,共有14条通路显著富集(P值<0.01,Q值<0.05)。(4)通过进一步对差异基因进行功能注释分析,共筛选出抗盐候选基因89个,其中,抗氧化基因24个,渗透调节物质基因22个,植物激素基因19个,蛋白激酶基因10个,转录因子基因14个。[结论]活性氧清除、渗透调节、植物激素、蛋白激酶及转录因子相关基因参与调控无瓣海桑盐逆境适应过程。

外文摘要：[Objective]To lay a molecular basis for functional verification experiment and genetic breeding of tree salinity tolerance, the genetic mechanisms was explored, and salt-related genes were identified for Sonneratia apetala Buch.-Ham. [Method]In this study, the root tissues were collected from a 1-year-old S.apetala treated with 500 mmol·L^(-1)NaCl for 0(control group) and 10 d(treatment group). Then transcriptome sequencing and bioinformatics analysis was performed based on the three-generation full-length transcriptome dataset of S. apetala. [Result](1) Compared with the control group, 14 401 genes were differentially expressed after salt treatment, of which 7 153 were up-regulated and 7 248 were down-regulated.(2) GO analysis found that a total of 11 068 differential genes were annotated in 47 GO items.(3) For KEGG enrichment analysis, a total of 6 189 differential expression genes were enriched to 134 pathways,of which 14 were significantly enriched(P-value <0.01, Q-value <0.05).(4) Further functional annotation analysis of the differentially expressed genes revealed a total of 89 genes was potential salt-related candidate genes. Among these, 24, 22, 19, 10 and 14 genes encoded enzymes or functional proteins referred to antioxidation, osmotic adjustment substances, plant hormones, protein kinase and transcription factors,respectively. [Conclusion]Genes relating to active oxygen scavenging, osmotic regulation, plant hormones, protein kinases and transcription factors participate in the regulation of salt stress adaptation in S.apetala.