文献类型：期刊文献

英文题名：Effects of T-2 toxin on the muscle proteins of shrimp (Litopenaeus vannamei) - a proteomics study

作者：Huang, Zhanrui[1];Wang, Yaling[1];Sun, Lijun[1];Wang, Xiaobo[1];Lu, Pengli[1];Liang, Guangming[1];Pang, Huanying[2];Wu, Qinghua[3];Gooneratne, Ravi[4];Zhao, Jian[5]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Product Proc & Safet, Key Lab Adv Proc Aquat Prod, Coll Food Sci & Technol,Guangdong Higher Educ Ins, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim, Coll Fisheries,Guangdong Higher Educ Ins, Zhanjiang, Peoples R China;[3]Yangtze Univ, Coll Life Sci, Jingzhou, Peoples R China;[4]Lincoln Univ, Dept Wine Food & Mol Biosci, Fac Agr & Life Sci, Lincoln, New Zealand;[5]Univ New South Wales, Sch Chem Engn, Sydney, NSW, Australia

年份：2020

卷号：100

期号：1

起止页码：119

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：SCI-EXPANDED(收录号：WOS:000488172300001)、、WOS

基金：This study was supported by the National Natural Science Foundation of China (NSFC) (No. 31871898) and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams of Shrimp, Department of Agricultural and Rural Affairs of Guangdong Province (No. 2019(1019)).

语种：英文

外文关键词：Litopenaeus vannamei; T-2 toxin; shrimp; protein quality; proteomics

外文摘要：BACKGROUND T-2 toxin (T-2) is a potent mycotoxin and a common contaminant of aquatic animal feed, posing a serious risk to health and aquatic animals. We investigated the effect of T-2 on shrimp muscle proteins using proteomics and conventional biochemical methods. Shrimp were fed a diet containing T-2 at 0-12.2 mg kg(-1) for 20 days, and changes to the muscle protein composition, ATPase activities, and the sulfhydryl (SH) content and hydrophobicity of actomyosin (AM) were determined. A proteomics study of the proteins was conducted with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional (2D) electrophoresis, and matrix-assisted laser desorption/ionization - time of flight mass spectrometry (MALDI-TOF/TOF MS). RESULTS Exposure to T-2 markedly affected the muscle protein composition of shrimp in a concentration-responsive manner that displayed a diphasic effect. At a low T-2 concentration (1.2 mg kg(-1)), the levels of three major muscle proteins (myofibrillar, sarcoplasmic, and stroma) increased but at higher concentrations they declined progressively. T-2 exposure also led to a breakdown of muscle proteins as evidenced by increases in alkali-soluble protein and the surface hydrophobicity (SoANS) of AM. Thirty differentially expressed proteins were detected, 12 of which showed a concentration-response relationship with T-2 exposure. Among them, 11 homologous proteins were identified by mass spectrometry (MS), with several being key enzymes in energy metabolism. CONCLUSION This study demonstrated that T-2 exposure at medium to high concentrations could significantly affect the protein composition and quality of shrimp muscle, and potentially some of its key metabolisms. One of the arginine kinases (spot 27) was particularly responsive to T-2 and could potentially be used as a biomarker protein for T-2 intoxication by shrimp. (c) 2019 Society of Chemical Industry