文献类型：期刊文献

英文题名：Identi?cation of a novel antisense RNA that regulates growth hormone receptor expression in chickens

作者：Lin, Shudai[1,2,3,4];Zhang, Zihao[1,2];Xie, Tingting[1,2];Hu, Bowen[1,2];Ruan, Zhuohao[1,2];Zhang, Li[5];Li, Congjun[3];Li, Charles[4];Luo, Wen[1,2];Nie, Qinghua[1,2];Zhang, Xiquan[1,2]

机构：[1]South China Agr Univ, Coll Anim Sci, Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China;[2]South China Agr Univ, Coll Anim Sci, Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China;[3]ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD USA;[4]ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD USA;[5]Guangdong Ocean Univ, Agr Coll, Zhanjiang, Peoples R China

年份：2019

卷号：16

期号：5

起止页码：626

外文期刊名：RNA BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000466373500003)、、Scopus(收录号：2-s2.0-85062041280)、WOS

基金：This work was funded by the China Agriculture Research System (CARS-41-G03), the National Natural Science Foundation of China (31672412), and the Graduate Student Overseas Study Program from South China Agricultural University (Grant No. 2017LHPY016).

语种：英文

外文关键词：Natural antisense transcript; chicken; GHR; GHBP; RNA-RNA duplex; fat deposition

外文摘要：Natural antisense transcripts (NATs) are widely present in mammalian genomes and act as pivotal regulator molecules of gene expression. However, studies on NATs in the chicken are relatively rare. We identi?ed a novel antisense transcript in the chicken, designated GHR-AS-EST, transcribed from the growth hormone receptor (GHR) locus, which encodes a well-known regulatory molecule of muscle development and fat deposition. GHR-AS-EST is predominantly expressed in the chicken liver and muscle tissues. GHR-AS-EST sequence conservation among vertebrates is weak. GHR-AS-EST forms an RNA-RNA duplex with GHBP to increase its stability, and regulates the expression of GHR sense transcripts at both the mRNA and protein levels. Further, GHR-AS-EST promotes cell proliferation by stimulating the expression of signaling factors in the JAK2/STAT pathway, and contributes to fat deposition via downregulating the expression of signaling factors in the JAK2/SOCS pathway in LMH hepatocellular carcinoma cells. We expect that the discovery of a NAT for a regulatory gene associated with cell proliferation and lipolysis will further our understanding of the molecular regulation of both muscle development and fat deposition.