文献类型：期刊文献

英文题名：Purification and identification of an ACE inhibitory peptide from the peptic hydrolysate of Acetes chinensis and its antihypertensive effects in spontaneously hypertensive rats

作者：Cao, Wenhong[1]; Zhang, Chaohua[1]; Hong, Pengzhi[1]; Ji, Hongwu[1]; Hao, Jiming[1]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong Province 524025, China

年份：2010

卷号：45

期号：5

起止页码：959

外文期刊名：International Journal of Food Science and Technology

收录：EI(收录号：20111213808827)、Scopus(收录号：2-s2.0-77955576619)

语种：英文

外文关键词：Shellfish - Blood pressure - Rats - Peptides

外文摘要：Acetes chinensis is a marine shrimp found in the coastal waters of China. The shrimp was hydrolysed by pepsin to prepare hydrolysates with angiotensin I-converting enzyme (ACE) inhibitory activity. The hydrolysate with the highest ACE inhibitory activity resulted from a 3-5 h incubation at 45°C and pH 2.5 with pepsin. Gel filtration and RP-HPLC were used to separate ACE inhibitory peptides from the hydrolysate. The gel filtration fraction of the hydrolysate with a molecular weight range from 1320 Da to 311 Da exerted the highest ACE inhibition activity. This fraction was separated by RP-HPLC into fifteen fractions, of which fraction F9 showed 92.7% of the ACE inhibition activity. Its peptide sequence was determined to be Leu-His-Pro. It showed a potent antihypertensive activity in spontaneously hypertensive rats. The results suggested that this peptide may be a potent ACE inhibitor which might be developed into a healthy food to lower blood pressure. ? 2010 The Authors. Journal compilation ? 2010 Institute of Food Science and Technology.