文献类型：期刊文献

中文题名：抵抗素成熟肽的制备及其对猪葡萄糖和脂肪代谢的影响

英文题名：Preparation of Resistin Mature Peptide and Its Effect on Glucose and Fat Metabolism in Pigs

作者：白翠玲[1];罗丹[1];刘艳芬[1];陈绍红[2];刘铀[2]

机构：[1]广东海洋大学农学院,广东湛江524088;[2]广东海洋大学生化中心,广东湛江524088

年份：2013

卷号：34

期号：11

起止页码：46

中文期刊名：动物医学进展

外文期刊名：Progress In Veterinary Medicine

收录：CSTPCD、、北大核心2011、CSCD_E2013_2014、北大核心、CSCD

基金：广东海洋大学人才引进项目(0612053)

语种：中文

中文关键词：抵抗素成熟肽;葡萄糖代谢;脂肪代谢;猪

外文关键词：resistin mature peptide;glucose metabolism;fat metabolism;pig

中文摘要：为探讨抵抗素成熟肽对猪葡萄糖和脂肪代谢的影响,将猪抵抗素成熟肽(MR)编码序列克隆至pET-32a(+),在大肠埃希菌中表达重组MR融合蛋白,并用Ni-NTA亲和层析纯化。经肠激酶消化切除融合蛋白,用Ni-NTA分离MR。随后将18头5月龄去势巴马香猪随机分为3个组,对照组肌肉注射生理盐水0.5mL,试验1组和2组分别肌肉注射20ng/kg和40ng/kg的MR,于注射后0、1、2、3、4、5h分别采血,检测血清葡萄糖及脂肪代谢相关指标。结果表明,重组MR融合蛋白分子质量为29ku,切除融合蛋白的MR分子质量为12ku,纯化的MR浓度约3mg/L。给予外源性MR后,试验组猪血清葡萄糖显著高于对照组(P<0.05);脂蛋白酯酶活性亦有升高的趋势,但组间差异不显著(P>0.05);两个试验组猪血清甘油三酯含量均显著降低,试验2组显著低于试验1组和对照组(P<0.05);试验2组猪血清游离脂肪酸含量显著高于试验1组和对照组(P<0.05)。由此可见,抵抗素能抑制组织细胞的葡萄糖摄入,并通过提高脂蛋白酯酶活性促进甘油三酯分解,在调节机体能量代谢与能量平衡中发挥重要作用。

外文摘要：The present work was aimed to further investigate the effects of resistin mature peptide on glu-cose and fat metabolism in pigs .The fragment encoding for resistin mature peptide（MR） was cloned into the expression vector pET-32a（＋ ） ,and expressed in Ecoli BL-21（DE3） .The recombinant MR fusion pro-tein was purified by Ni-NTA affinity chromatography .Then the fusion peptide was cleaved from the re-combinant MR fusion protein by enterokinase digestion ,and the purified MR was isolated by Ni-NTA af-finity chromatography subsequently .18 5-month-old castrated Bama pigs were randomly divided into 3 groups ,the pigs in control group received nomal saline 0 .5 mL intramuscularly ,and the animals in experi-mental groups respectively received 20 and 40 ng MR per kilogram body weight ,blood samples were col-lected at 0 ,1 ,2 ,3 ,4 ,5 h after administration ,and the biochemical parameters associated glucose and fat metabolism were detected .The results showed that the recombinant MR fusion protein with molecular weight of 29 ku ,MR cleaved fusion protein with molecular weight of 12 ku were detected by SDS-PAGE , and the concentration of purified MR was 3 mg/L .The results also revealed that the serum glucose levels of the pigs in the experimental groups were significantly increased （P0 .05）;the serum triglyceride contents of the animals in the experimental groups were signif-icantly decreased ,moreover ,the serum triglyceride level of the pigs in experimental group 2 was signifi-cantly lower than that of control and experimental group 1（P〈0 .05）;and the free fatty acid content of test group 2 was also significantly higher than that of control and test group 1（P〈0 .05） .The results a-bove suggested that the glucose intake of porcine histocytes was inhibited and the triglycerides decomposi-tion was promoted along with increased serum lipoprotein lipase activity owing to high dose of MR admin-istration ,resistin may play an important role in the regulation of energy metabolism and homeostasis of an-imals .