文献类型：期刊文献

英文题名：Induction of jasmonate signalling regulators MaMYC2s and their physical interactions with MaICE1 in methyl jasmonate-induced chilling tolerance in banana fruit

作者：Zhao, Ming-Lei[1];Wang, Jun-Ning[1,3];Shan, Wei[1];Fan, Jia-Geng[1];Kuang, Jian-Fei[1];Wu, Ke-Qiang[2];Li, Xue-Ping[1];Chen, Wei-Xin[1];He, Fang-Yuan[1];Chen, Jian-Ye[1];Lu, Wang-Jin[1]

机构：[1]S China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangdong Key Lab Postharvest Sci, Coll Hort Sci, Guangzhou 510642, Guangdong, Peoples R China;[2]Chinese Acad Sci, State Key Lab Plant Resources Conservat & Sustain, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China

年份：2013

卷号：36

期号：1

起止页码：30

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:000311974000003)、、Scopus(收录号：2-s2.0-84870507080)、WOS

基金：We thank Dr Yuhai Cui (Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre) and Prof. Zhixiang Chen (Department of Botany and Plant Pathology, Purdue University) for their critical revising of the manuscript and suggestions. We are also grateful to Prof. Wei-dong Huang (College of Food Science and Nutrientional Engineering, China Agricultural University), Prof. Jorg Kudla (Institut fur Biologie und Biotechnologie der Pflanzen, Universitat Munster) and Prof. Seiichiro Hasezawa (Department of Integrated Biosciences, The University of Tokyo) for the generous gift of pEZS-NL vector, BiFC vectors and tobacco BY2 suspension cells, respectively. This work was supported in part by the National Natural Science Foundation of China (Grant no. 30972068), China Agriculture Research System (Grant No. CARS-32-02A), Guangdong Modern Agricultural Industry Technology System (Grant No. LNSG2010-12) and Guangdong Province Undergraduate Innovative Experiment Project.

语种：英文

外文关键词：ICE-CBF; MYC2; protein-protein interaction

外文摘要：MYC2, a basic helix-loop-helix (bHLH) transcription factor, is a key regulator in the activation of jasmonate (JA) response. However, the molecular details of MYC2 involving in methyl jasmonate (MeJA)-induced chilling tolerance of fruit remain largely unclear. In the present work, two MYC2 genes, MaMYC2a and MaMYC2b, and one homolog of the inducer of the C-repeat-binding factor (CBF) gene, MaICE1 were isolated and characterized from banana fruit. MaMYC2s and MaICE1 were found to be all localized in the nucleus. In addition, the proline-rich domain (PRD) and the acidic domain (AD) in the N-terminus were important for the transcriptional activation of MaMYC2 in yeast cells. Unlike MaICE1's constitutive expression, MaMYC2a and MaMYC2b were induced rapidly following MeJA treatment during cold storage. Moreover, proteinprotein interaction analysis confirmed that MaMYC2s interacted with MaICE1. The expression of ICE-CBF cold-responsive pathway genes including MaCBF1, MaCBF2, MaCOR1, MaKIN2, MaRD2 and MaRD5 was also significantly induced by MeJA. Taken together, our work provides strong evidence that MaMYC2 is involved in MeJA-induced chilling tolerance in banana fruit through physically interacting and likely functionally coordinating with MaICE1, revealing a novel mechanism for ICE1 in response to cold stress as well as during development of induced chilling tolerance.