文献类型：期刊文献

英文题名：Comparative Analyses of Zebrafish Anxiety-Like Behavior Using Conflict-Based Novelty Tests

作者：Kysil, Elana V.[1];Meshalkina, Darya A.[1];Frick, Erin E.[2];Echevarria, David J.[2,3];Rosemberg, Denis B.[3,4];Maximino, Caio[3,5];Lima, Monica Gomes[3,6];Abreu, Murilo S.[7];Giacomini, Ana C.[7];Barcellos, Leonardo J. G.[3,7,8];Song, Cai[9,10];Kalueff, Allan V.[1,3,9,11,12]

机构：[1]St Petersburg State Univ, Inst Translat Biomed, St Petersburg 199034, Russia;[2]Univ Southern Mississippi, Dept Psychol, Hattiesburg, MS USA;[3]Int Zebrafish Neurosci Res Consortium ZNRC, Slidell, LA USA;[4]Univ Fed Santa Maria, Grad Program Biol Sci Toxicol Biochem, Santa Maria, RS, Brazil;[5]Fed Univ Southern & Southeastern Para UNIFESSPA, Lab Neurosci & Behav Frederico Guilherme Graeff, Ctr Biol & Hlth Sci, Inst Hlth & Biol Studies, Maraba, Brazil;[6]Univ State Para UEPA, Maraba, Brazil;[7]Univ Passo Fundo, Postgrad Program Bioexperimentat, Passo Fundo, Brazil;[8]Fed Univ Santa Maria UFSM, Postgrad Program Pharmacol, Santa Maria, RS, Brazil;[9]Guangdong Ocean Univ, Res Inst Marine Drugs & Nutr, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[10]China Med Univ Hosp, Grad Inst Neural & Cognit Sci, Taichung, Taiwan;[11]Ural Fed Univ, Ekaterinburg, Russia;[12]ZENEREI Res Ctr, Slidell, LA USA

年份：2017

卷号：14

期号：3

起止页码：197

外文期刊名：ZEBRAFISH

收录：SCI-EXPANDED(收录号：WOS:000402566000001)、、Scopus(收录号：2-s2.0-85020182570)、WOS

基金：The study was coordinated through the International Zebrafish Neuroscience Research Consortium (ZNRC), and this collaboration was supported by St. Petersburg State University Intramural Research program (DMM, EVK, AVK), Ural Federal University (AVK), Guangdong Ocean University (CS, AVK), and the University of Passo Fundo (LJGB), CNPq grant 470260/2013 (LJGB) and CNPq research fellowships 301992/2014-2 (LJGB) and 307595/2015-3 (DBR). The funders had no involvement in the study design, data collection or analysis, and article preparation. AVK is the Chair of ZNRC, and his research is supported by the Russian Foundation for Basic Research (RFBR) grant 16-04-00851. The authors thank Mr. Rodrigo Zanandrea (University of Passo Fundo, Brazil) for his assistance with zebrafish cortisol analyses.

语种：英文

外文关键词：zebrafish; anxiety-like behavior; behavioral phenotyping; the novel tank test; the light-dark test

外文摘要：Modeling of stress and anxiety in adult zebrafish (Danio rerio) is increasingly utilized in neuroscience research and central nervous system (CNS) drug discovery. Representing the most commonly used zebrafish anxiety models, the novel tank test (NTT) focuses on zebrafish diving in response to potentially threatening stimuli, whereas the light-dark test (LDT) is based on fish scototaxis (innate preference for dark vs. bright areas). Here, we systematically evaluate the utility of these two tests, combining meta-analyses of published literature with comparative in vivo behavioral and whole-body endocrine (cortisol) testing. Overall, the NTT and LDT behaviors demonstrate a generally good cross-test correlation in vivo, whereas meta-analyses of published literature show that both tests have similar sensitivity to zebrafish anxiety-like states. Finally, NTT evokes higher levels of cortisol, likely representing a more stressful procedure than LDT. Collectively, our study reappraises NTT and LDT for studying anxiety-like states in zebrafish, and emphasizes their developing utility for neurobehavioral research. These findings can help optimize drug screening procedures by choosing more appropriate models for testing anxiolytic or anxiogenic drugs.