文献类型：期刊文献

英文题名：A Stable Delivery System for Meretrix meretrix Derived Immunomodulatory Peptide (QLNWD): Fabrication and Characterization of Glycosylated Protein Nanoparticle

作者：Wu, Wanyi[1];Wu, Zhixuan[1];Cai, Jiamin[1];Cao, Wenhong[1,2];Lin, Haisheng[1,2];Gao, Jialong[1,2];Fan, Xiuping[1,2];Zheng, Huina[1,2];Qin, Xiaoming[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Natl Res & Dev Branch Ctr Shellfish Proc Zhanjiang, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[3]Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Zhanjiang 524000, Peoples R China

年份：2025

卷号：23

期号：10

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:001601903400001)、、Scopus(收录号：2-s2.0-105020029356)、WOS

基金：This work was funded by China Agriculture Research System (CARS-49), the Shellfish & Algae Industry Innovation Team of Guangdong Modern Agricultural Technology System (2024CXTD23), the National Key R&D Program of China (2024YFD2401805), and the Talent Team Introduction Program of Zhanjiang Bay Laboratory (2025) under the project "Development of 'Guangfu No.1' Oyster Line and Key Technologies for Enhancing the Zhanjiang Oyster Industrial Chain" (ZJW202415).

语种：英文

外文关键词：marine immunomodulatory peptide; Meretrix meretrix; Maillard reaction; self-assembly; glycosylated protein nanoparticle; stabilization

外文摘要：In this study, nanoparticles prepared by the heat-induced self-assembly of bovine serum albumin-dextran conjugates (BSA-DX) were utilized as an effective delivery system for the immunoregulatory peptide Gln-Leu-Asn-Trp-Asp (QLNWD) from Meretrix meretrix. The effects of dry-heating duration on the fabrication and characteristics of glycoprotein nanoparticles (GBA NPs) were investigated. Stable GBA NPs (110.84 nm) were obtained after 9 h of dry-heating. Depending on the addition sequence of QLNWD, the QLNWD-loaded nanoparticles were categorized into two types: pre-loading and post-loading. The two strategies were evaluated based on physicochemical characterization, colloidal stability, and RAW264.7 macrophage uptake. Results showed that upon QLNWD incorporation, both pre-loading NPs and post-loading NPs exhibited spherical morphology, with particle sizes decreasing to 105.51 nm and 94.27 nm, respectively. The encapsulation efficiency of pre-loading NPs for QLNWD was higher (87.74%), and the co-localization ability between post-loading NPs and QLNWD was stronger (Pearson's correlation coefficient = 0.95). In vitro simulated gastrointestinal digestion experiments showed that QLNWD bioaccessibility increased to 47.5% and 42.7% for pre-loaded and post-loaded NPs, respectively. Compared to free QLNWD, NP encapsulation significantly enhanced the uptake of QLNWD by macrophages. Thus, GBA NPs, particularly those prepared by the pre-loading method, are considered promising delivery systems for marine bioactive peptides.