文献类型：期刊文献

英文题名：Pathogenicity and transcriptomic exploration of Vibrio fortis in Penaeus monodon

作者：Dai, Linxin[1];Chen, Peixun[1];Hou, Danqing[1];Wang, Yue[1];Zhou, Yinhuan[1,2];Sun, Chengbo[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol A, Zhanjiang, Guangdong, Peoples R China

年份：2023

卷号：142

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001084448900001)、、Scopus(收录号：2-s2.0-85173022656)、WOS

基金：This work was supported by the Science and Technology Program of Guangdong Province (Grant No.2021B0202020003) , and the project of the innovation team for the innovation and utilization of Economic Animal Germplasm in the South China Sea (Grant No. 2021KCXTD026) .

语种：英文

外文关键词：Vibrio fortis; Penaeus monodon; Transcriptomic analysis; Antimicrobial susceptibility testing; Medial lethal concentration

外文摘要：In this study, a strain (recorded as Y6) was isolated from the biofloc pool, its DNA was extracted for 16S rDNA sequencing and compared in the NCBI database, and it was identified as Vibrio fortis. The V. fortis was activated, cultured, and artificially injected into Penaeus monodon to observe the symptoms and calculate the semi-lethal concentration (LC50). It was found that the symptoms of the red leg, an empty stomach, and enlarged hepato-pancreas of P. monodon after infection with V. fortis. The LC50 was 4.00 x 107, 2.24 x 107, 1.82 x 107, 1.41 x 107, 7.52 x 106 and 3.31 x 106 CFU/mL at 16, 24, 32, 48, 128, and 144 hpi, respectively. The K-B disk method was used to detect the sensitivity of V. fortis to various antibiotic drugs. V. fortis resisted Ampicillin, Piperacillin, Cefazolin, Cephalothin and Cefoxitin. Highly sensitive to Polymyxin B, Tobramycin, Gentamicin, Cefepime, Cefoperazone and Streptomycin. To explore the molecular response mechanism of V. fortis infection in P. monodon, the hepatopancreas of P. monodon infected with V. fortis at 24 and 48 hpi by transcriptome sequencing, and a total of 347 DEGs were obtained (214 up-regulated DEGs and 133 down-regulated DEGs). In the KEGG pathway enrichment analysis of DEGs, significant changes were found in genes and signaling pathways related to immune system and substance metabolism, including NOD-like receptor signaling pathways, Toll and Imd signaling pathways, C-type lectin receptor signaling pathways and pyruvate metabolism. This study initially revealed the immune response of P. monodon to V. fortis infection from the molecular level and provided a reference for further understanding of the study and control of the vibriosis of shrimp.