文献类型：期刊文献

中文题名：低温胁迫对军曹鱼幼鱼血清生化指标、肝脏抗氧化酶活性及凋亡相关基因表达量的影响

英文题名：Effects of Low-Temperature Stress on Serum Biochemical,Antioxidant Enzymes Activities and Apoptosis-Related Gene Expression in Liver of Juvenile Cobia(Rachycentron canadum)

作者：李豫[1];黄建盛[1];陈有铭[2];温震威[1];欧光海[1];黄鉴鹏[1];蒋鑫涛[1];郭潮安[1];马骞[1];陈刚[1,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]广东蓝粮种业有限公司,广东湛江524000;[3]广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088

年份：2022

卷号：42

期号：5

起止页码：18

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、北大核心、北大核心2020

基金：财政部和农业农村部国家现代农业产业技术体系专项资金资助(CARS-47);国家重点研发计划项目(2020YFD0900200);广东省科技计划项目(2016B020201009)。

语种：中文

中文关键词：军曹鱼;低温胁迫;生理生化指标;抗氧化酶;凋亡相关基因

外文关键词：Rachycentron canadum;low-temperature stress;biochemical index;antioxidant enzyme;apoptosis-related gene

中文摘要：【目的】探究低温对军曹鱼(Rachycentron canadum)幼鱼生理机能的影响。【方法】将军曹鱼幼鱼分别置于28(对照组)、25、21、18℃4种温度条件下,于0、4、7 d取样检测血清生化指标、肝脏抗氧化酶活性和凋亡相关基因表达量。【结果】(1)军曹鱼血清葡萄糖(GLU)含量在4、7 d时28℃组显著高于温度处理组(P<0.05);甘油三酯(TG)含量在0、7 d时21、18℃组显著高于28、25℃组(P<0.05);总胆固醇(T-CHO)含量在21、18℃组所有时间点均显著低于28、25℃组(P<0.05);总蛋白(TP)含量仅在7 d时21、18℃组显著低于28、25℃组(P<0.05);谷丙转氨酶(ALT)活性在0、4 d时18℃组含量显著高于其他组(P<0.05);谷草转氨酶(AST)活性在0 d时28℃组显著低于温度处理组(P<0.05);碱性磷酸酶(AKP)在4、7 d时21、18℃组与28、25℃组有显著差异(P<0.05)。(2)肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性在4、7 d时21、18℃组显著低于28、25℃组(P<0.05);谷胱甘肽过氧化物酶(GPx)活性在4 d时21、18℃组显著低于28、25℃组(P<0.05),在7 d时则显著升高(P<0.05)。丙二醛(MDA)含量21℃和18℃组均高于28、25℃组且在7 d时差异显著(P<0.05)。(3)肝脏凋亡相关基因bax、caspase-9、caspase-3、p53、mdm2表达量在温度处理组中均高于28℃组,Bcl-2在0、7 d时18℃组显著低于其他组(P<0.05)。【结论】低温胁迫显著降低军曹鱼幼鱼血清抗氧化相关酶活性,诱导肝脏组织中凋亡相关基因表达,从而造成氧化损伤,促进细胞凋亡。

外文摘要：【Objective】To investigate the effects of low-temperature stress on physiological function of juvenile cobia(Rachycentron canadum),【Method】Juvenile cobia were put in four aquaculture tanks whose water temperatures were 28℃(control),25℃,21℃and 18℃respectively to study the changes of serum biochemical,antioxidant enzyme activities and apoptosis-related gene expression in the liver of cobia at different time(0,4 and 7 d).【Results】The results in the current study indicated that:(1)The serum glucose(GLU)content of cobia at 4 and 7 d in the 28℃group was significantly higher than that in temperature treatment groups(P<0.05);The content of triglyceride(TG)was extensively higher in the 21 and 18℃groups than in the 28 and 25℃groups(P<0.05)at 0 and 7 d;Compared with the 28 and 25℃groups,the total cholesterol(T-CHO)content showed a significant decrease in the 21 and 18℃groups(P<0.05)at 0,4 and 7 d.The total protein(TP)content considerably lowered in the 21 and 18℃groups,compared to the 28 and 25℃groups after 7 d(P<0.05).The alanine aminotransferase(ALT)activity was considerably higher in the 18℃group than other groups at 0 and 4 d,while the activity of aspartate aminotransferase(AST)in the 28℃group decreased significantly versus temperature treatment groups at 0 d(P<0.05).There were significant variations in serum alkaline phosphatase(AKP)between the two groups(21 and 18℃)and the other two groups(28 and 25℃)at the times of 4 and 7 d(P<0.05).(2)The activities of superoxide dismutase(SOD)and catalase(CAT)in the 21 and 18℃groups were significantly lower than those in the 28 and 25℃groups at 4 and 7 d in the liver(P<0.05).Hepatic glutathione peroxidase(GPx)activity in the 21 and 18℃groups significantly decreased at 4 d,but significantly increased at 7 d,compared to the 28 and 25℃groups(P<0.05).The content of malondialdehyde(MDA)was significantly higher in the 21 and 18℃groups than that in the 28 and 25℃groups at 7 d(P<0.05).(3)The expression levels of hepatic apoptosis-related genes(i.e.bax,caspase-9,caspase-3,p53 and mdm2)up-regulated in the 28℃group than temperature treatment groups,while bcl-2 down-regulated considerably in the 18℃group,compared to other groups at 0 and 7 d(P<0.05).【Conclusion】Low-temperature stress could significantly lower the activities of antioxidation-related enzymes and induce the expression of apoptosis-related genes,thereby leading to the oxidative damage and the promotion of cellular apoptosis in cobia.