文献类型：期刊文献

英文题名：Distribution and Transfer of Antibiotic Resistance Genes in Coastal Aquatic Ecosystems of Bohai Bay

作者：Jia, Lei[1];Liu, Hao[1];Zhao, Na[2];Deng, Qiuxia[2];Zhu, Chunhua[2];Zhang, Bo[1,2]

机构：[1]Tianjin Fisheries Res Inst, Tianjin 300200, Peoples R China;[2]Guangdong Ocean Univ, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Fisheries Coll, Zhanjiang 524000, Peoples R China

年份：2022

卷号：14

期号：6

外文期刊名：WATER

收录：SCI-EXPANDED(收录号：WOS:000774404000001)、、EI(收录号：20221912073072)、Scopus(收录号：2-s2.0-85129440671)、WOS

基金：This research was funded by grants from the Tianjin Natural Science Foundation (17JCQNJC15000), China Agriculture Research System of MOF and MARA (CARS-47-Z01) and the modern industrial technology system in Tianjin ITTMRS2022001.

语种：英文

外文关键词：antibiotic resistance genes; seawater; soil; fish intestines; horizontal transfer

外文摘要：Antibiotic resistance genes (ARGs) are abundant in diverse ecosystems and the resistome may constitute a health threat for humans and animals. It is necessary to uncover ARGs and the accumulation mechanisms from different environmental sources. Various habitats, such as soil, seawater and fish intestines, could overflow a considerable amount of ARGs and the horizontal transfer of ARGs may occur in these environments. Thus, we assessed the composition and abundance of ARGs in seawater, soil and intestinal tracts of Cynoglossus semilaevis collected from different sites in Bohai Bay (China), including a natural area and three fish farms, through a high-throughput qPCR array. In total, 243 ARGs were uncovered, governing the resistance to aminoglycoside, multidrug, beta-lactamase, macrolide lincosamide streptomycin B (MLSB), chloramphenicol, sulfonamide, tetracycline, vancomycin and other antibiotics. The action mechanisms of these ARGs were mainly antibiotic deactivation, efflux pump and cellular protection. Importantly, similar ARGs were detected in different samples but show dissimilar enrichment levels. ARGs were highly enriched in the fish farms compared to the natural sea area, with more genes detected, while some ARGs were detected only in the natural sea area samples, such as bacA-02, tetL-01 and ampC-06. Regarding sample types, water samples from all locations shared more ARGs in common and held the highest average level of ARGs detected than in the soil and fish samples. Mobile genetic elements (MGEs) were also detected in three sample types, in the same trend as ARGs. This is the first study comparing the resistome of different samples of seawater, soil and intestines of C. semilaevis. This study contributes to a better understanding of ARG dissemination in water sources and could facilitate the effective control of ARG contamination in the aquatic environment.