文献类型：期刊文献

英文题名：Identification and characteristics of aptamers against inactivated Vibrio alginolyticus

作者：Zheng, Jiang[1];Tang, Xuemin[1];Wu, Renxie[2];Yan, Qingpi[1];Tang, Hua[1];Luo, Jiawei[1];Niu, Sufang[2];Qu, Yuankai[2];Sun, Liwei[2]

机构：[1]Jimei Univ, Coll Fisheries, Fujian Prov Key Lab Marine Fishery Resources & Ec, Xiamen 361021, Fujian, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China

年份：2015

卷号：64

期号：2

起止页码：1138

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000360773500088)、、EI(收录号：20154201416265)、Scopus(收录号：2-s2.0-84944318708)、WOS

基金：This work was supported by grants from the Key Science and Technology Program of Fujian Province (2013N0025), the Open Research Fund Program of Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment (C221502), the Science and Technology Program of Xiamen Southern Oceanographic Center (No.14PYY050SF03), the Science and Technology Program from Education Department of Fujian Province (JB10096), and the Science Foundation of Jimei University (ZQ2011002).

语种：英文

外文关键词：Detection; Affinity constant (Kd); Secondary structure

外文摘要：Vibrio alginolyticus is an opportunistic pathogen that can infect many aquatic animals and cause economical losses to aquaculture and the food industry. Therefore, sensitive and rapid detection of V. alginolyticus is urgently needed. In the present paper, three aptamers were proved to have good specificity towards inactivated V. alginolyticus, and they could distinguish inactivated V. alginolyticus from inactivated Vibrio harveyi, Vibrio parahaemolyticus, Vibrio anguillarum, Edwardsiella tarda, Aeromonas hydrophila and Escherichia coli. Incubating heat- and formaldehyde-treated cells with aptamers #7, #8, and #23, washes with binding buffer, heat-induced release of bound aptamers, and application of the polymerase chain reaction to amplify the aptamers allowed the detection of 10, 10 and 10(3) cells/ml, respectively. Aptamer #8 had good affinity with both live and inactivated V. alginolyticus. The combination of aptamers #8 and #7, or aptamers #8 and #23, could distinguish live V. alginolyticus from inactivated V. alginolyticus based on their different affinities with the microorganism. The putative secondary structures were determined for these aptamers using software RNAstructure 5.3 and the affinity constants (Kds) 28.39 +/- 11.46, 12.82 +/- 6.00 and 46.89 +/- 15.87 nM, were calculated respectively by employing their saturation curves. The results demonstrated that an aptamer-based method can be used for the detection of V. alginolyticus. (C) 2015 Elsevier Ltd. All rights reserved.