文献类型：期刊文献

中文题名：构树叶提取物抑菌活性的初步观察

英文题名：Preliminary Observation of Antibacterial Activity of Broussonetia papyrifera Leaf Extracts

作者：刘晓军[1];刘铀[2];陈绍红[2];刘艳芬[1]

机构：[1]广东海洋大学农学院,广东湛江524088;[2]广东海洋大学生化中心,广东湛江524088

年份：2013

卷号：19

期号：19

起止页码：283

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD、、北大核心2011、北大核心

基金：广东省农业厅科技项目(0809035)

语种：中文

中文关键词：构树叶;提取;抑菌活性;最小抑菌浓度

外文关键词：Broussonetialpapyrifera leaf; extract; antibacteria activity; MIC

中文摘要：目的:研究构树叶提取物的抑菌活性。方法:采用有机溶剂萃取、硅胶柱层析等方法从构树叶提取活性成分,观察其对金黄色葡萄球菌、多杀性巴氏埃希菌、大肠杆菌、沙门氏菌和鸭疫里默氏杆菌等病原菌的抑制作用、最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:水、70%乙醇和50%丙酮提取物对鸭疫里默氏杆菌外的病原菌均有较好的抑菌活性,水提取物对其他几种菌的MIC分别为6.75,25,6.75,12.5 g·L-1,有机溶剂分步萃取构树叶水提取物,抑菌成分主要存在于乙酸乙酯和正丁醇萃取物中;用硅胶柱层析从乙酸乙酯和正丁醇萃取物中均分离到3个具有抑菌活性的组分。结论:构树叶中含有多种抑菌成分,其理化性质、抑菌活性和抑菌机制各不相同;进一步分离、鉴定上述抑菌成分并测定其化学结构可望为开发新型抗菌药物提供依据。

外文摘要：Objective： In order to study the antibacterial activity of the Broussonetia papyrifera leaf extracts. Method： The active components of B. papyrifera leaf were extracted by organic solvent, and silica gel column chromatography. The antibacterial effect in vitro of the extracts above on several pathogenic bacteria stains such as Staphylococcus aureus, Pasteurellamultocida, Escherichia coli, Salmonolla sp and Riemerella anatipestifer were observed, and the minimum inhibitory concentration （MIC）, minimal bactericidal concentration （MBC） were also determined. Result： The result indicated that water, 70% ethanol and 50% acetone extracts of B. papyrifera leaf extracts showed better antibacterial activity, and the MIC of the water extract agaist S. aureus, Pasteurellamultocida, E. coli and Salmonolla sp were 6.75, 25, 6.75, 12.5 g·L^-1 respectively. The bacteriostatic constituents mainly exist in ethyl acetate and n-butanol extract as the B. papyrifera leaf water extract were extracted by organic solvent step by step. Moreover, 3 components had antibacterial activity agaist the pathogenic bacteria were isolated from ethyl acetate and n-butanol extract respectively. Conclusion： B. papyrifera leaf contains a variety of antibacterial ingredients, which vary in physicochemical properties, antibacterial activity and mechanism; further study on separation, identification and chemical structure determination of the compounds is expected to provide the basis for development of new antimicrobial drugs.